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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GRP78/BIP Monoclonal antibody, PBS Only

GRP78/BIP Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D6F7

Cat no : 66574-1-PBS

Synonyms

BIP, GRP 78, GRP78, GRP78/BIP, Heat shock 70 kDa protein 5, HSPA5, MIF2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRP78/BIP Monoclonal antibody (66574-1-Ig)

    WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GRP78/BIP Monoclonal antibody (66574-1-Ig)

    WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GRP78/BIP Monoclonal antibody (66574-1-Ig)

    WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GRP78/BIP Monoclonal antibody (66574-1-Ig)

    WB analysis using 66574-1-Ig (same clone as 66574-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of SGC-7901 cells using GRP78/BIP Monoclonal antibody (66574-1-Ig)

    IP experiment of SGC-7901 using 66574-1-Ig (same clone as 66574-1-PBS)

    IP result of anti-GRP78/BIP (IP:66574-1-Ig, 5ug; Detection:66574-1-Ig 1:3000) with SGC-7901 cells lysate 2200 ug. This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GRP78/BIP Monoclonal antibody (66574-1-Ig)

    IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GRP78/BIP Monoclonal antibody (66574-1-Ig)

    IHC staining of human liver cancer using 66574-1-Ig (same clone as 66574-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GRP78/BIP Monoclonal antibody (66574-1-Ig)

    IHC staining of human breast cancer using 66574-1-Ig (same clone as 66574-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GRP78/BIP Monoclonal antibody (66574-1-Ig)

    IHC staining of human breast cancer using 66574-1-Ig (same clone as 66574-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GRP78/BIP Monoclonal antibody (66574-1-Ig)

    IHC staining of human colon cancer using 66574-1-Ig (same clone as 66574-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GRP78/BIP Monoclonal antibody (66574-1-Ig)

    IHC staining of human colon cancer using 66574-1-Ig (same clone as 66574-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66574-1-Ig (GRP78,BIP antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GRP78/BIP Monoclonal antibody (66574-1-Ig)

    IF Staining of HeLa using 66574-1-Ig (same clone as 66574-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66574-1-Ig (GRP78/BIP antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66574-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66574-1-PBS targets GRP78/BIP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GRP78/BIP fusion protein Ag2188 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Calculated molecular weight 78 kDa
Observed molecular weight 70-78 kDa
GenBank accession numberBC020235
Gene symbol GRP78/BIP
Gene ID (NCBI) 3309
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.