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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GRP94 Monoclonal antibody, PBS Only (Capture)

GRP94 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4G7C7

Cat no : 60012-2-PBS

Synonyms

HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRP94 Monoclonal antibody (60012-2-Ig)

    WB analysis using 60012-2-Ig (same clone as 60012-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60012-2-Ig (GRP94 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50013-1

    Sandwich ELISA standard curve of MP50013-1

    Sandwich ELISA standard curve of MP50013-1, GRP94 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60012-2-PBS. Detection antibody: HRP-conjugated 60012-1-PBS. Standard: Ag1439. Range: 0.156-20 ng/mL..

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GRP94 Monoclonal antibody (60012-2-Ig)

    IHC staining of human colon cancer using 60012-2-Ig (same clone as 60012-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60012-2-Ig (GRP94 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GRP94 Monoclonal antibody (60012-2-Ig)

    IHC staining of human colon cancer using 60012-2-Ig (same clone as 60012-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60012-2-Ig (GRP94 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GRP94 Monoclonal antibody (60012-2-Ig)

    IHC staining of human pancreas cancer using 60012-2-Ig (same clone as 60012-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60012-2-Ig (GRP94 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GRP94 Monoclonal antibody (60012-2-Ig)

    IHC staining of human pancreas cancer using 60012-2-Ig (same clone as 60012-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60012-2-Ig (GRP94 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GRP94 Monoclonal antibody (60012-2-Ig)

    IF Staining of HepG2 using 60012-2-Ig (same clone as 60012-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GRP94 antibody (60012-2-Ig, Clone: 4G7C7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using GRP94 Monoclonal antibody (60012-2-Ig)

    IF Staining of A431 using 60012-2-Ig (same clone as 60012-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 60012-2-Ig (GRP94 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using GRP94 Monoclonal antibody (60012-2-Ig)

    FC experiment of HeLa using 60012-2-Ig (same clone as 60012-2-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GRP94 (60012-2-Ig, Clone:4G7C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60012-2-PBS targets GRP94 as part of a matched antibody pair:

MP50013-1: 60012-2-PBS capture and 60012-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GRP94 fusion protein Ag1439 相同性解析による交差性が予測される生物種
Full Name heat shock protein 90kDa beta (Grp94), member 1
Calculated molecular weight 96 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC009195
Gene symbol GRP94
Gene ID (NCBI) 7184
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270).