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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GRP94 Recombinant antibody

GRP94 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

6K5

Cat no : 82805-1-RR

Synonyms

HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRP94 Recombinant antibody (82805-1-RR)

    WB analysis using 82805-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82805-1-RR (GRP94 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82805-1-RR

    Affinity and Kinetic Characterization of 82805-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82805-1-RR against Human GRP94 were performed. The affinity constant is 1.30 nM.

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82805-1-RR targets GRP94 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GRP94 fusion protein Ag6344 相同性解析による交差性が予測される生物種
Full Name heat shock protein 90kDa beta (Grp94), member 1
Calculated molecular weight 92 kDa
Observed molecular weight 94-100 kDa
GenBank accession numberBC066656
Gene symbol GRP94
Gene ID (NCBI) 7184
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GRP94 antibody 82805-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols