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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GRP94 Recombinant antibody

GRP94 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

6G15

Cat no : 82805-2-RR

Synonyms

HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRP94 Recombinant antibody (82805-2-RR)

    WB analysis using 82805-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82805-2-RR (GRP94 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GRP94 Recombinant antibody (82805-2-RR)

    IHC staining of human breast cancer using 82805-2-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82805-2-RR (GRP94 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GRP94 Recombinant antibody (82805-2-RR)

    IHC staining of human breast cancer using 82805-2-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82805-2-RR (GRP94 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GRP94 Recombinant antibody (82805-2-RR)

    IHC staining of human lung cancer using 82805-2-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82805-2-RR (GRP94 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GRP94 Recombinant antibody (82805-2-RR)

    IHC staining of human lung cancer using 82805-2-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82805-2-RR (GRP94 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human normal colon using GRP94 Recombinant antibody (82805-2-RR)

    IHC staining of human normal colon using 82805-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82805-2-RR (GRP94 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82805-2-RR

    Affinity and Kinetic Characterization of 82805-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82805-2-RR against Human GRP94 were performed. The affinity constant is 101 nM.

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6400
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82805-2-RR targets GRP94 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GRP94 fusion protein Ag6344 相同性解析による交差性が予測される生物種
Full Name heat shock protein 90kDa beta (Grp94), member 1
Calculated molecular weight 92 kDa
Observed molecular weight 95-100 kDa
GenBank accession numberBC066656
Gene symbol GRP94
Gene ID (NCBI) 7184
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.

Protocols

Product Specific Protocols
WB protocol for GRP94 antibody 82805-2-RRDownload protocol
IHC protocol for GRP94 antibody 82805-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols