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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GRPEL1 Polyclonal antibody

GRPEL1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12720-1-AP

Synonyms

Mt-GrpE#1, Mt GrpE#1, HMGE, GREPEL1

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using GRPEL1 Polyclonal antibody (12720-1-AP)

    WB analysis of MCF-7 using 12720-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12720-1-AP (GRPEL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GRPEL1 Polyclonal antibody (12720-1-AP)

    WB analysis using 12720-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12720-1-AP (GRPEL1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using GRPEL1 Polyclonal antibody (12720-1-AP)

    IP experiment of MCF-7 using 12720-1-AP

    IP result of anti-GRPEL1 (IP:12720-1-AP, 4ug; Detection:12720-1-AP 1:10000) with MCF-7 cells lysate 1000 ug.

  • Immunohistochemistry (IHC) staining of human bowen disease using GRPEL1 Polyclonal antibody (12720-1-AP)

    IHC staining of human bowen disease using 12720-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12720-1-AP (GRPEL1 antibody) at dilution of 1:2400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using GRPEL1 Polyclonal antibody (12720-1-AP)

    IHC staining of human bowen disease using 12720-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12720-1-AP (GRPEL1 antibody) at dilution of 1:2400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GRPEL1 Polyclonal antibody (12720-1-AP)

    IF Staining of HepG2 using 12720-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GRPEL1 antibody (12720-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GRPEL1 Polyclonal antibody (12720-1-AP)

    IF Staining of HepG2 using 12720-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GRPEL1 antibody (12720-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inMCF-7 cells, HEK-293T cells
Positive IP detected inMCF-7 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

12720-1-AP targets GRPEL1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRPEL1 fusion protein Ag3415 相同性解析による交差性が予測される生物種
Full Name GrpE-like 1, mitochondrial (E. coli)
Calculated molecular weight 217 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC024242
Gene symbol GRPEL1
Gene ID (NCBI) 80273
RRIDAB_2114331
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GRPEL1 antibody 12720-1-APDownload protocol
IHC protocol for GRPEL1 antibody 12720-1-APDownload protocol
IF protocol for GRPEL1 antibody 12720-1-APDownload protocol
IP protocol for GRPEL1 antibody 12720-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.

Authors - Chun-Shik Shin
View Article
humanWB

Nat Commun

Protein import motor complex reacts to mitochondrial misfolding by reducing protein import and activating mitophagy

Authors - Jonas Benjamin Michaelis
View Article
humanWB

EBioMedicine

The LIV-1-GRPEL1 axis adjusts cell fate during anti-mitotic agent-damaged mitosis.

Authors - Pingbo Chen
View Article