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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GS28 Recombinant antibody, PBS Only (Capture)

GS28 Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242552G12

Cat no : 84971-3-PBS

Synonyms

GOSR1, GOS-28, Golgi SNAP receptor complex member 1, 28 kDa Golgi SNARE protein, 28 kDa cis-Golgi SNARE p28

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GS28 Recombinant antibody (84971-3-RR)

    WB analysis using 84971-3-RR (same clone as 84971-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84971-3-RR (GS28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GS28 Recombinant antibody (84971-3-RR)

    IHC staining of human stomach cancer using 84971-3-RR (same clone as 84971-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84971-3-RR (GS28 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GS28 Recombinant antibody (84971-3-RR)

    IHC staining of human stomach cancer using 84971-3-RR (same clone as 84971-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84971-3-RR (GS28 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using GS28 Recombinant antibody (84971-3-RR)

    IF Staining of NIH/3T3 using 84971-3-RR (same clone as 84971-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using GS28 antibody (84971-3-RR, Clone: 242552G12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84971-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01719-2

    Cytometric bead array standard curve of MP01719-2

    Cytometric bead array standard curve of MP01719-2, GS28 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84971-3-PBS. Detection antibody: 84971-4-PBS. Standard: Ag9021. Range: 0.625-80 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84971-3-PBS targets GS28 as part of a matched antibody pair:

MP01719-2: 84971-3-PBS capture and 84971-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GS28 fusion protein Ag9021 相同性解析による交差性が予測される生物種
Full Name golgi SNAP receptor complex member 1
Calculated molecular weight28.6 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC012620
Gene symbol GS28
Gene ID (NCBI) 9527
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GS28, also known as GOSR1, is a 28-kDa Golgi SNARE protein that participates in ER-Golgi and intra-Golgi transport. GS28 is considered to be a core component of the Golgi SNARE complex. (PMID: 8638159)