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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GSDMA Recombinant antibody (82920-2-RR)

    WB analysis using 82920-2-RR

    Daudi cells were subjected to SDS PAGE followed by western blot with 82920-2-RR (GSDMA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GSDMA Recombinant antibody (82920-2-RR)

    IF Staining of HeLa using 82920-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSDMA antibody (82920-2-RR, Clone: 230161C5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Affinity and Kinetic Characterization of 82920-2-RR

    Affinity and Kinetic Characterization of 82920-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82920-2-RR against Human GSDMA were performed. The affinity constant is below 1 pM.

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GSDMA Recombinant antibody

GSDMA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82920-2-RR
Clone No.230161C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Gasdermin-A, Gasdermin-1, gasdermin A, Gasdermin 1, FKSG9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inDaudi cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82920-2-RR targets GSDMA in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GSDMA fusion protein Ag24407 相同性解析による交差性が予測される生物種
Full Name gasdermin A
Calculated molecular weight 445 aa, 49 kDa
Observed molecular weight49 kDa
GenBank accession numberBC109197
Gene Symbol GSDMA
Gene ID (NCBI) 284110
RRIDAB_3670656
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96QA5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Gasdermins, a family of five pore-forming proteins (GSDMA-GSDME) in humans expressed predominantly in the skin, mucosa and immune sentinel cells, are key executioners of inflammatory cell death (pyroptosis), which recruits immune cells to infection sites and promotes protective immunity. GSDMA was predicted to enable phosphatidylinositol-4,5-bisphosphate binding activity, phosphatidylinositol-4-phosphate binding activity, and phosphatidylserine binding activity. Involved in apoptotic process.

Protocols

Product Specific Protocols
WB protocol for GSDMA antibody 82920-2-RRDownload protocol
IF protocol for GSDMA antibody 82920-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols