Featured Product
KD/KO Validated

GSDMB Polyclonal antibody

Name: GSDMB Polyclonal antibody
Brand: Proteintech
SKU: 12885-1-AP

GSDMB Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12885-1-AP

Synonyms

gasdermin B, Gasdermin like protein, GSDMB, GSDML, PP4052, PRO2521

Validation Data Gallery

Filter:

AllWBIHC

WB analysis of PC-3 using 12885-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 12885-1-AP (GSDMB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 12885-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 12885-1-AP (GSDMB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 12885-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12885-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12885-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12885-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12885-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12885-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12885-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12885-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12885-1-AP (GSDMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 12885-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected in

human liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 4 publications below
IF	See 1 publications below

Product Information

12885-1-AP targets GSDMB in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GSDMB fusion protein Ag3895 相同性解析による交差性が予測される生物種
Full Name	gasdermin B
Calculated molecular weight	411 aa, 47 kDa
Observed molecular weight	47 kDa
GenBank accession number	BC025682
Gene symbol	GSDMB
Gene ID (NCBI)	55876
RRID	AB_2114762
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for GSDMB antibody 12885-1-AP	Download protocol
IHC protocol for GSDMB antibody 12885-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC,WB	Front Immunol Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma Authors - Liangmin Fu KD Validated View Article
human	WB, IHC	Int J Biol Sci USP24-GSDMB complex promotes bladder cancer proliferation via activation of the STAT3 pathway. Authors - Haiqing He View Article
human	IHC	Front Oncol Novel Pyroptosis-Related Gene Signatures Identified as the Prognostic Biomarkers for Bladder Carcinoma. Authors - Jia You View Article
human	WB	Toxicol Appl Pharmacol Schisandrin B induces HepG2 cells pyroptosis by activating NK cells mediated anti-tumor immunity Authors - Anping Song View Article
human	IHC,IF	Cell Death Dis Clinical-mediated discovery of pyroptosis in CD8+ T cell and NK cell reveals melanoma heterogeneity by single-cell and bulk sequence Authors - Ying Zhang View Article
human	WB	J Allergy Clin Immunol A functional splice variant associated with decreased asthma risk abolishes the ability of gasdermin B to induce epithelial cell pyroptosis. Authors - Ronald A Panganiban View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSDMB Polyclonal antibody

GSDMB Polyclonal Antibody for IHC, ELISA

Validation Data Gallery

WB analysis of PC-3 using 12885-1-AP

WB analysis of COLO 320 using 12885-1-AP

IHC staining of human liver cancer using 12885-1-AP

IHC staining of human liver cancer using 12885-1-AP

IHC staining of human colon cancer using 12885-1-AP

IHC staining of human colon cancer using 12885-1-AP

IHC staining of human colon cancer using 12885-1-AP

IHC staining of human colon cancer using 12885-1-AP

IHC staining of human liver cancer using 12885-1-AP

IHC staining of human liver cancer using 12885-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma

USP24-GSDMB complex promotes bladder cancer proliferation via activation of the STAT3 pathway.

Novel Pyroptosis-Related Gene Signatures Identified as the Prognostic Biomarkers for Bladder Carcinoma.

Schisandrin B induces HepG2 cells pyroptosis by activating NK cells mediated anti-tumor immunity

Clinical-mediated discovery of pyroptosis in CD8+ T cell and NK cell reveals melanoma heterogeneity by single-cell and bulk sequence

A functional splice variant associated with decreased asthma risk abolishes the ability of gasdermin B to induce epithelial cell pyroptosis.