- Featured Product
- KD/KO Validated
GSDMD Polyclonal antibody
GSDMD Polyclonal Antibody for WB, IP, IF, FC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IP, IF, FC, IHC, ELISA
Conjugate
Unconjugated
201
Cat no : 20770-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, NIH/3T3 cells, Jurkat cells, C6 cells, rat liver tissue, rat brain tissue |
| Positive IP detected in | THP-1 cells |
| Positive IF detected in | HeLa cells |
| Positive FC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 160 publications below |
| IHC | See 42 publications below |
| IF | See 48 publications below |
| FC | See 1 publications below |
Product Information
20770-1-AP targets GSDMD in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | Human, rat, mouse, pig, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GSDMD fusion protein Ag14757 相同性解析による交差性が予測される生物種 |
| Full Name | gasdermin D |
| Calculated molecular weight | 484 aa, 53 kDa |
| Observed molecular weight | 50-53 kDa |
| GenBank accession number | BC008904 |
| Gene symbol | GSDMD |
| Gene ID (NCBI) | 79792 |
| RRID | AB_10696319 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GSDMD antibody 20770-1-AP | Download protocol |
| IF protocol for GSDMD antibody 20770-1-AP | Download protocol |
| IP protocol for GSDMD antibody 20770-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores.
| ||
ACS Nano Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration. | ||
Acta Pharm Sin B Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis. | ||
Acta Pharm Sin B A high-throughput Gaussia luciferase reporter assay for screening potential gasdermin E activators against pancreatic cancer | ||
Cell Res Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion. | ||
J Exp Med Protein kinase D at the Golgi controls NLRP3 inflammasome activation.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Isha (Verified Customer) (03-02-2021) | Bands were so clear. Could not refrain myself from writing this review.
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