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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSDMD Polyclonal antibody

GSDMD Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30531-1-AP

Synonyms

Gasdermin-D, C-terminal, Gasdermin-D, gasderminD, Gasdermin domain-containing protein 1, gasdermin D

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using GSDMD (C-terminal) Polyclonal antibody (30531-1-AP)

    WB analysis of THP-1 using 30531-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 30531-1-AP (GSDMD (C-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using GSDMD Polyclonal antibody (30531-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 30531-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30531-1-AP (GSDMD antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using GSDMD Polyclonal antibody (30531-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 30531-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30531-1-AP (GSDMD antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inTHP-1 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30531-1-AP targets GSDMD in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSDMD fusion protein Ag33627 相同性解析による交差性が予測される生物種
Full Name gasdermin D
Calculated molecular weight 484 aa, 53 kDa
Observed molecular weight53 kDa, 43 kDa
GenBank accession numberBC008904
Gene symbol GSDMD
Gene ID (NCBI) 79792
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.

Protocols

Product Specific Protocols
WB protocol for GSDMD antibody 30531-1-APDownload protocol
IHC protocol for GSDMD antibody 30531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols