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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSG1 Polyclonal antibody

GSG1 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 15258-1-AP

Synonyms

UNQ709/PRO1360, Germ cell-specific gene 1 protein, germ cell associated 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GSG1 Polyclonal antibody (15258-1-AP)

    WB analysis using 15258-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15258-1-AP (GSG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using GSG1 Polyclonal antibody (15258-1-AP)

    IF Staining of mouse testis using 15258-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using GSG1 antibody (15258-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse testis tissue
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15258-1-AP targets GSG1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSG1 fusion protein Ag7200 相同性解析による交差性が予測される生物種
Full Name germ cell associated 1
Calculated molecular weight 37 kDa
Observed molecular weight39 kDa
GenBank accession numberBC001796
Gene symbol GSG1
Gene ID (NCBI) 83445
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GSG1 causes the redistribution of PAPOLB from the cytosol to the endoplasmic reticulum. It has eight isoforms with MW 31-33 kDa and 36-40 kDa.

Protocols

Product Specific Protocols
WB protocol for GSG1 antibody 15258-1-APDownload protocol
IF protocol for GSG1 antibody 15258-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols