Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, K-562 cells, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55454-1-AP targets GSG2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | germ cell associated 2 (haspin) |
| Calculated molecular weight | 88 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | NM_031965 |
| Gene Symbol | GSG2 |
| Gene ID (NCBI) | 83903 |
| RRID | AB_3086451 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TF76 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Haploid germ cell-specific nuclear protein kinase (Haspin, alias GSG2) is an atypical eukaryotic protein kinase that was for a long time considered an inactive pseudokinase due to the low degree of structural homology of GSG2 with the 'classical' protein kinases (PMID: 29698892). GSG2 is a serine/threonine kinase that associates with chromosomes and phosphorylates threonine 3 of histone 3 during mitosis (PMID: 32439830).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GSG2 antibody 55454-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |