• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSG2 Recombinant antibody

GSG2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240887E11

Cat no : 84098-1-RR

Synonyms

HASPIN, H haspin, 240887E11

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of A431 cells using GSG2 Recombinant antibody (84098-1-RR)

    IF Staining of A431 using 84098-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using GSG2 antibody (84098-1-RR, Clone: 240887E11 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using GSG2 Recombinant antibody (84098-1-RR)

    FC experiment of A431 using 84098-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Gsg2 Recombinant Antibody (84098-1-RR, Clone:240887E11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

☆ 3/31（月）まで！二次抗体 & FlexAbleが30%オフ！ >> 免疫アッセイサポートキャンペーン

Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84098-1-RR targets GSG2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name germ cell associated 2 (haspin)
Calculated molecular weight 88 kDa
GenBank accession numberNM_031965
Gene symbol GSG2
Gene ID (NCBI) 83903
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Haploid germ cell-specific nuclear protein kinase (Haspin, alias GSG2) is an atypical eukaryotic protein kinase that was for a long time considered an inactive pseudokinase due to the low degree of structural homology of GSG2 with the 'classical' protein kinases (PMID: 29698892). GSG2 is a serine/threonine kinase that associates with chromosomes and phosphorylates threonine 3 of histone 3 during mitosis (PMID: 32439830).

Protocols

Product Specific Protocols
IF protocol for GSG2 antibody 84098-1-RRDownload protocol
FC protocol for GSG2 antibody 84098-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols