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- KD/KO Validated
GSK3B Polyclonal antibody
GSK3B Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 15113-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse ovary tissue, HEK-293 cells, HeLa cells, A549 cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 9 publications below |
Product Information
15113-1-AP targets GSK3B in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GSK3B fusion protein Ag7101 相同性解析による交差性が予測される生物種 |
| Full Name | glycogen synthase kinase 3 beta |
| Calculated molecular weight | 433 aa, 48 kDa |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | BC000251 |
| Gene symbol | GSK3B |
| Gene ID (NCBI) | 2932 |
| RRID | AB_10640527 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .This antibody recognize the 2 isoforms of GSK3B.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GSK3B antibody 15113-1-AP | Download protocol |
| IHC protocol for GSK3B antibody 15113-1-AP | Download protocol |
| IP protocol for GSK3B antibody 15113-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Oncol The Role of miR-640: A Potential Suppressor in Breast Cancer via Wnt7b/β-catenin Signaling Pathway. | ||
J Ethnopharmacol Entada phaseoloides extract suppresses hepatic gluconeogenesis via activation of the AMPK signaling pathway. | ||
Biochem Biophys Res Commun Perilipin 5 ameliorates high-glucose-induced podocyte injury via Akt/GSK-3β/Nrf2-mediated suppression of apoptosis, oxidative stress, and inflammation. | ||
Biochem Biophys Res Commun NOL4L, a novel nuclear protein, promotes cell proliferation and metastasis by enhancing the PI3K/AKT pathway in ovarian cancer. | ||
Microvasc Res Luteolin alleviates ischemia/reperfusion injury-induced no-reflow by regulating Wnt/β-catenin signaling in rats. | ||
Cell Biol Int Identification and analysis of glycogen synthase kinase 3 beta1 interactome. |
