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GSK3B Polyclonal antibody

Name: GSK3B Polyclonal antibody
Brand: Proteintech
SKU: 22104-1-AP
Rating: 4.00 (1 reviews)

GSK3B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

358

Reviews (1)

Cat no : 22104-1-AP

Synonyms

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK-3B, GSK3B 216P, gsk3beta, GSK-3beta, GSK3β

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 22104-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 22104-1-AP
WB result of GSK3B antibody (22104-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSK3B transfected A549 cells.
WB analysis of rat brain using 22104-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
WB analysis of mouse kidney using 22104-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 22104-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of LNCaP using 22104-1-AP
LNCaP cells were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 22104-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 22104-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 22104-1-AP (GSK3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 22104-1-AP
IP result of anti-GSK3B (IP:22104-1-AP, 4ug; Detection:22104-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human lung cancer using 22104-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22104-1-AP (GSK3B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 22104-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22104-1-AP (GSK3B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 22104-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 22104-1-AP (GSK3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 22104-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 22104-1-AP (GSK3B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 22104-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 22104-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 22104-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 22104-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human prostate hyperplasia using 22104-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human prostate hyperplasia using 22104-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human prostate hyperplasia using 22104-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human prostate hyperplasia using 22104-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 22104-1-AP (GSK3B antibody) at dilution of 1:50 (under 40x lens).
IF Staining of MCF-7 using 22104-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GSK3B antibody (22104-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of MCF-7 using 22104-1-AP
Immunofluorescent analysis of MCF-7 cells, using GSK3B antibody 22104-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 22104-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, A549 cells, mouse brain tissue, mouse kidney tissue, mouse ovary tissue, LNCaP cells, mouse testis tissue, rat brain tissue, PC-3 cells, mouse thymus tissue, rat thymus tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human lung cancer tissue, human prostate hyperplasia tissue, human testis tissue, rat colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 10 publications below
WB	See 347 publications below
IHC	See 15 publications below
IF	See 12 publications below
IP	See 7 publications below
CoIP	See 6 publications below
RIP	See 1 publications below

Product Information

22104-1-AP targets GSK3B in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken, zebrafish, bovine, hamster
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GSK3B fusion protein Ag17320 相同性解析による交差性が予測される生物種
Full Name	glycogen synthase kinase 3 beta
Calculated molecular weight	433 aa, 48 kDa
Observed molecular weight	46-48 kDa
GenBank accession number	BC000251
Gene symbol	GSK3B
Gene ID (NCBI)	2932
RRID	AB_2878997
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase.GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution. This antibody recognize the C-terminal of GSK3B.

Protocols

Product Specific Protocols
WB protocol for GSK3B antibody 22104-1-AP	Download protocol
IHC protocol for GSK3B antibody 22104-1-AP	Download protocol
IF protocol for GSK3B antibody 22104-1-AP	Download protocol
IP protocol for GSK3B antibody 22104-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature CFIm25 links alternative polyadenylation to glioblastoma tumour suppression. Authors - Chioniso P Masamha View Article
human	WB	Mol Cancer Hypoxia downregulated miR-4521 suppresses gastric carcinoma progression through regulation of IGF2 and FOXM1. Authors - Shan Xing View Article
human	WB	Cell Rep Med Development of an orally bioavailable CDK12/13 degrader and induction of synthetic lethality with AKT pathway inhibition Authors - Yu Chang View Article
human	WB	Nat Commun Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response. Authors - Wenjun Wang View Article
zebrafish	WB	Nat Commun ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling. Authors - Xiaoyun Sun View Article
rat	WB	Hepatology Shp2 promotes liver cancer stem cell expansion by augmenting β-catenin signaling and predicts chemotherapeutic response of patients. Authors - Daimin Xiang View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Tom (Verified Customer) (12-15-2020)

HEK293 lysates - 10ug total protein. Blocked in 5% BSA before primary antibody (1:1000) overnight at 4 degrees. Anti-rabbit HRP (1:10,000) for 1 hour at RT.

Applications: Western Blot
Primary Antibody Dilution: 1:1000
Cell Tissue Type: HEK293T

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSK3B Polyclonal antibody

GSK3B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 22104-1-AP

WB analysis of A549 using 22104-1-AP

WB analysis of rat brain using 22104-1-AP

WB analysis of mouse kidney using 22104-1-AP

WB analysis of mouse ovary using 22104-1-AP

WB analysis of LNCaP using 22104-1-AP

WB analysis of mouse brain using 22104-1-AP

WB analysis of mouse testis using 22104-1-AP

IP experiment of mouse brain using 22104-1-AP

IHC staining of human lung cancer using 22104-1-AP

IHC staining of human lung cancer using 22104-1-AP

IHC staining of rat colon using 22104-1-AP

IHC staining of rat colon using 22104-1-AP

IHC staining of human testis using 22104-1-AP

IHC staining of human testis using 22104-1-AP

IHC staining of human testis using 22104-1-AP

IHC staining of human testis using 22104-1-AP

IHC staining of human prostate hyperplasia using 22104-1-AP

IHC staining of human prostate hyperplasia using 22104-1-AP

IHC staining of human prostate hyperplasia using 22104-1-AP

IHC staining of human prostate hyperplasia using 22104-1-AP

IF Staining of MCF-7 using 22104-1-AP

IF Staining of MCF-7 using 22104-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.

Hypoxia downregulated miR-4521 suppresses gastric carcinoma progression through regulation of IGF2 and FOXM1.

Development of an orally bioavailable CDK12/13 degrader and induction of synthetic lethality with AKT pathway inhibition

Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.

ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling.

Shp2 promotes liver cancer stem cell expansion by augmenting β-catenin signaling and predicts chemotherapeutic response of patients.

Reviews