GSK3B Polyclonal antibody
GSK3B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 24198-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, HeLa cells, PC-12 cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | Ethacrynic acid treated HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 3 publications below |
Product Information
24198-1-AP targets GSK3B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GSK3B fusion protein Ag21438 相同性解析による交差性が予測される生物種 |
Full Name | glycogen synthase kinase 3 beta |
Calculated molecular weight | 433 aa, 48 kDa |
Observed molecular weight | 46 kDa |
GenBank accession number | BC000251 |
Gene symbol | GSK3B |
Gene ID (NCBI) | 2932 |
RRID | AB_2879451 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen Affinity purified |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for GSK3B antibody 24198-1-AP | Download protocol |
IHC protocol for GSK3B antibody 24198-1-AP | Download protocol |
IF protocol for GSK3B antibody 24198-1-AP | Download protocol |
IP protocol for GSK3B antibody 24198-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Cardiovasc Med Vascular Smooth Muscle FTO Promotes Aortic Dissecting Aneurysms via m6A Modification of Klf5. | ||
Tumour Biol Downregulation of β-catenin blocks fibrosis via Wnt2 signaling in human keloid fibroblasts. | ||
Medicine (Baltimore) Wnt2 knock down by RNAi inhibits the proliferation of in vitro-cultured human keloid fibroblasts. | ||
Biomed Res Int Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling. |