GSK3B Polyclonal antibody

GSK3B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24198-1-AP

Synonyms

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK-3B, GSK3B 216P, gsk3beta


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Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, PC-12 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inEthacrynic acid treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

24198-1-AP targets GSK3B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSK3B fusion protein Ag21438 相同性解析による交差性が予測される生物種
Full Name glycogen synthase kinase 3 beta
Calculated molecular weight 433 aa, 48 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC000251
Gene symbol GSK3B
Gene ID (NCBI) 2932
RRIDAB_2879451
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GSK3B antibody 24198-1-APDownload protocol
IHC protocol for GSK3B antibody 24198-1-APDownload protocol
IF protocol for GSK3B antibody 24198-1-APDownload protocol
IP protocol for GSK3B antibody 24198-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Cardiovasc Med

Vascular Smooth Muscle FTO Promotes Aortic Dissecting Aneurysms via m6A Modification of Klf5.

Authors - Dong Ma
humanWB

Tumour Biol

Downregulation of β-catenin blocks fibrosis via Wnt2 signaling in human keloid fibroblasts.

Authors - Yumei Cai
humanWB

Medicine (Baltimore)

Wnt2 knock down by RNAi inhibits the proliferation of in vitro-cultured human keloid fibroblasts.

Authors - Yumei Cai

Biomed Res Int

Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling.

Authors - Xueyun Chen