GSK3B Polyclonal antibody

Name: GSK3B Polyclonal antibody
Brand: Proteintech
SKU: 24198-1-AP

GSK3B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 24198-1-AP

Synonyms

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK-3B, GSK3B 216P, gsk3beta

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of mouse brain using 24198-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 24198-1-AP (GSK3B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 24198-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24198-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 24198-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 24198-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 24198-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 24198-1-AP (GSK3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 24198-1-AP
IP result of anti-GSK3B (IP:24198-1-AP, 4ug; Detection:24198-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human testis using 24198-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24198-1-AP (GSK3B Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 24198-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24198-1-AP (GSK3B Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 24198-1-AP
Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 24198-1-AP (GSK3B antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Cat No. 24198-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, HeLa cells, PC-12 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Ethacrynic acid treated HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below

Product Information

24198-1-AP targets GSK3B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GSK3B fusion protein Ag21438 相同性解析による交差性が予測される生物種
Full Name	glycogen synthase kinase 3 beta
Calculated molecular weight	433 aa, 48 kDa
Observed molecular weight	46 kDa
GenBank accession number	BC000251
Gene symbol	GSK3B
Gene ID (NCBI)	2932
RRID	AB_2879451
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for GSK3B antibody 24198-1-AP	Download protocol
IHC protocol for GSK3B antibody 24198-1-AP	Download protocol
IF protocol for GSK3B antibody 24198-1-AP	Download protocol
IP protocol for GSK3B antibody 24198-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Front Cardiovasc Med Vascular Smooth Muscle FTO Promotes Aortic Dissecting Aneurysms via m6A Modification of Klf5. Authors - Dong Ma View Article
human	WB	Tumour Biol Downregulation of β-catenin blocks fibrosis via Wnt2 signaling in human keloid fibroblasts. Authors - Yumei Cai View Article
human	WB	Medicine (Baltimore) Wnt2 knock down by RNAi inhibits the proliferation of in vitro-cultured human keloid fibroblasts. Authors - Yumei Cai View Article
		Biomed Res Int Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling. Authors - Xueyun Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSK3B Polyclonal antibody

GSK3B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of mouse brain using 24198-1-AP

WB analysis using 24198-1-AP

WB analysis of HeLa using 24198-1-AP

WB analysis of mouse brain using 24198-1-AP

IP experiment of mouse brain using 24198-1-AP

IHC staining of human testis using 24198-1-AP

IHC staining of human testis using 24198-1-AP

IF Staining of HeLa using 24198-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Vascular Smooth Muscle FTO Promotes Aortic Dissecting Aneurysms via m6A Modification of Klf5.

Downregulation of β-catenin blocks fibrosis via Wnt2 signaling in human keloid fibroblasts.

Wnt2 knock down by RNAi inhibits the proliferation of in vitro-cultured human keloid fibroblasts.

Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling.