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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GSK3B Monoclonal antibody (67329-1-Ig)

    WB analysis using 67329-1-Ig (same clone as 67329-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67329-1-Ig (GSK3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67329-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GSK3B Monoclonal antibody (67329-1-Ig)

    WB analysis using 67329-1-Ig (same clone as 67329-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67329-1-Ig (GSK3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67329-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using GSK3B Monoclonal antibody (67329-1-Ig)

    WB analysis of pig brain using 67329-1-Ig (same clone as 67329-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67329-1-Ig (GSK3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67329-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GSK3B Monoclonal antibody (67329-1-Ig)

    IHC staining of human breast cancer using 67329-1-Ig (same clone as 67329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67329-1-Ig (GSK3B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67329-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GSK3B Monoclonal antibody (67329-1-Ig)

    IHC staining of human breast cancer using 67329-1-Ig (same clone as 67329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67329-1-Ig (GSK3B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67329-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GSK3B Monoclonal antibody (67329-1-Ig)

    IHC staining of human liver cancer using 67329-1-Ig (same clone as 67329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67329-1-Ig (GSK3B antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67329-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GSK3B Monoclonal antibody (67329-1-Ig)

    IHC staining of human liver cancer using 67329-1-Ig (same clone as 67329-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67329-1-Ig (GSK3B antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67329-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GSK3B Monoclonal antibody (67329-1-Ig)

    IF Staining of HeLa using 67329-1-Ig (same clone as 67329-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSK3B antibody (67329-1-Ig, Clone: 2E9E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67329-1-PBS in a different storage buffer formulation.

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GSK3B Monoclonal antibody, PBS Only

GSK3B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67329-1-PBS
Clone No.2E9E4

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

GSK3 beta, GSK-3 beta, GSK 3 beta, EC:2.7.11.26, EC:2.7.11.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67329-1-PBS targets GSK3B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GSK3B fusion protein Ag17320 相同性解析による交差性が予測される生物種
Full Name glycogen synthase kinase 3 beta
Calculated molecular weight 433 aa, 48 kDa
Observed molecular weight 46-48 kDa
GenBank accession numberBC000251
Gene Symbol GSK3B
Gene ID (NCBI) 2932
RRIDAB_2882588
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49841
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .