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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSK3B Recombinant antibody

GSK3B Recombinant Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

4N21

Cat no : 82061-1-RR

Synonyms

GSK3 beta, GSK-3 beta, GSK 3 beta, EC:2.7.11.26, EC:2.7.11.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GSK3B Recombinant antibody (82061-1-RR)

    WB analysis using 82061-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82061-1-RR (GSK3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using GSK3B Recombinant antibody (82061-1-RR)

    IP experiment of HeLa using 82061-1-RR

    IP result of anti-GSK3B (IP:82061-1-RR, 4ug; Detection:82061-1-RR 1:5000) with HeLa cells lysate 1720 ug.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using GSK3B Recombinant antibody (82061-1-RR)

    IHC staining of mouse skin using 82061-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 82061-1-RR (GSK3B antibody) at dilution of 1:450 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using GSK3B Recombinant antibody (82061-1-RR)

    IHC staining of mouse skin using 82061-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 82061-1-RR (GSK3B antibody) at dilution of 1:450 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82061-1-RR

    Affinity and Kinetic Characterization of 82061-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82061-1-RR against Human GSK3B were performed. The affinity constant is 7.36 pM.

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, A549 cells, Jurkat cells, PC-12 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:225-1:900
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82061-1-RR targets GSK3B in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GSK3B fusion protein Ag17320 相同性解析による交差性が予測される生物種
Full Name glycogen synthase kinase 3 beta
Calculated molecular weight 433 aa, 48 kDa
Observed molecular weight~45 kDa
GenBank accession numberBC000251
Gene symbol GSK3B
Gene ID (NCBI) 2932
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase.GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis.

Protocols

Product Specific Protocols
WB protocol for GSK3B antibody 82061-1-RRDownload protocol
IHC protocol for GSK3B antibody 82061-1-RRDownload protocol
IP protocol for GSK3B antibody 82061-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols