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Gelsolin Polyclonal antibody

Gelsolin Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 11644-2-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

GSN, Actin-depolymerizing factor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA549 cells, U2OS cells, HeLa cells
Positive IP detected inU2OS cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11644-2-AP targets Gelsolin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Gelsolin fusion protein Ag2239 相同性解析による交差性が予測される生物種
Full Name gelsolin (amyloidosis, Finnish type)
Calculated molecular weight 782 aa, 86 kDa
Observed molecular weight 87-93 kDa
GenBank accession numberBC026033
Gene Symbol Gelsolin
Gene ID (NCBI) 2934
RRIDAB_2295090
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06396
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Gelsolin (GSN) is an actin binding protein that regulates actin-severing/depolymerizing in a calcium dependent manner, and acts as a multifunctional regulator for cell metabolism and survival. Gelsolin exists in two isoforms: a cytoplasmic gelsolin (cGSN; 80-90 kDa) and a plasma gelsolin (pGSN; 93 kDa), differing at N terminal residues. Decreased levels of pGSN are strongly associated with human diseases, such as, acute liver injury, major trauma, myocardial infarction, inflammation, lung injury, rheumatoid arthritis, hemodialysis, multiple sclerosis, Alzheimer's disease. Plasma gelsolin can interact with actin and form a complex of 130 kDa (PMID: 6330060, 3030756).

Protocols

Product Specific Protocols
WB protocol for Gelsolin antibody 11644-2-APDownload protocol
IHC protocol for Gelsolin antibody 11644-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Adv Healthc Mater

Modification of PLGA Scaffold by MSC-Derived Extracellular Matrix Combats Macrophage Inflammation to Initiate Bone Regeneration via TGF-β-Induced Protein.

Authors - Moyuan Deng
WB

Genes Dev

Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein

Authors - Megan L Norris
humanWB,IF

Am J Cancer Res

3'untranslated regions (3'UTR) of Gelsolin mRNA displays anticancer effects in non-small cell lung cancer (NSCLC) cells.

Authors - Xiaohui Liu
humanWB, IHC

Gene

Role of gelsolin in cell proliferation and invasion of human hepatocellular carcinoma cells.

Authors - Biao Deng
mouseWB

Biomed Pharmacother

RACK1 promotes the proliferation, migration and invasion capacity of mouse hepatocellular carcinoma cell line in vitro probably by PI3K/Rac1 signaling pathway.

Authors - Wu Jun J
humanWB

Oncotarget

Correlation of five secretory proteins with the nasopharyngeal carcinoma metastasis and the clinical applications.

Authors - Ya Tao
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