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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    WB analysis of HepG2 using 16379-1-AP

    WB result of GSNOR,ADH5 antibody (16379-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSNOR,ADH5 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    WB analysis using 16379-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    WB analysis of human liver using 16379-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    WB analysis of human brain using 16379-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    WB analysis of human testis using 16379-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    IP experiment of mouse liver using 16379-1-AP

    IP result of anti-GSNOR,ADH5 (IP:16379-1-AP, 4ug; Detection:16379-1-AP 1:5000) with mouse liver tissue lysate 2400 ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    IHC staining of human pancreas cancer using 16379-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    IHC staining of human pancreas cancer using 16379-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    IHC staining of human endometrial cancer using 16379-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 25x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    IHC staining of human testis using 16379-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    IHC staining of human testis using 16379-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    IF Staining of HepG2 using 16379-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSNOR,ADH5 antibody (16379-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    FC experiment of HepG2 using 16379-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GSNOR,ADH5 Polyclonal antibody (16379-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • Featured Product
  • KD/KO Validated

GSNOR,ADH5 Polyclonal antibody

GSNOR,ADH5 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 16379-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Synonyms

ADH5, Alcohol dehydrogenase class-3, ADHX, ADH3, ADH 5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, human brain tissue, human liver tissue, human testis tissue, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16379-1-AP targets GSNOR,ADH5 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSNOR,ADH5 fusion protein Ag9360 相同性解析による交差性が予測される生物種
Full Name alcohol dehydrogenase 5 (class III), chi polypeptide
Calculated molecular weight 374 aa, 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC014665
Gene Symbol ADH5
Gene ID (NCBI) 128
RRIDAB_2289298
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11766
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).

Protocols

Product Specific Protocols
WB protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
IHC protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
IF protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
IP protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
FC protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Gene Copy Number and Post-Transductional Mechanisms Regulate TRAP1 Expression in Human Colorectal Carcinomas.

Authors - Michele Pietrafesa
  • KD Validated
View Article