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  • Featured Product
  • KD/KO Validated

GSNOR,ADH5 Polyclonal antibody

GSNOR,ADH5 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16379-1-AP

Synonyms

ADH5, Alcohol dehydrogenase class-3, ADHX, ADH3, ADH 5

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    WB analysis of HepG2 using 16379-1-AP

    WB result of GSNOR,ADH5 antibody (16379-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSNOR,ADH5 transfected HepG2 cells.

  • Western Blot (WB) analysis of mouse liver tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    WB analysis of mouse liver using 16379-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    WB analysis of mouse brain using 16379-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    WB analysis of mouse heart using 16379-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    WB analysis of human liver using 16379-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    WB analysis of human brain using 16379-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    WB analysis of human testis using 16379-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    IP experiment of mouse liver using 16379-1-AP

    IP result of anti-GSNOR,ADH5 (IP:16379-1-AP, 3ug; Detection:16379-1-AP 1:800) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    IHC staining of human testis using 16379-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    IHC staining of human testis using 16379-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    IHC staining of human endometrial cancer using 16379-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 25x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    IHC staining of human pancreas cancer using 16379-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    IHC staining of human pancreas cancer using 16379-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GSNOR,ADH5 Polyclonal antibody (16379-1-AP)

    IF Staining of HepG2 using 16379-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse liver tissue, human liver tissue, human brain tissue, human testis tissue, mouse heart tissue, HepG2 cells, mouse brain tissue
Positive IP detected inmouse liver tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16379-1-AP targets GSNOR,ADH5 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSNOR,ADH5 fusion protein Ag9360 相同性解析による交差性が予測される生物種
Full Name alcohol dehydrogenase 5 (class III), chi polypeptide
Calculated molecular weight 374 aa, 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC014665
Gene symbol ADH5
Gene ID (NCBI) 128
RRIDAB_2289298
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).

Protocols

Product Specific Protocols
WB protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
IHC protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
IF protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
IP protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
FC protocol for GSNOR,ADH5 antibody 16379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Gene Copy Number and Post-Transductional Mechanisms Regulate TRAP1 Expression in Human Colorectal Carcinomas.

Authors - Michele Pietrafesa
  • KD Validated
View Article