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  • Featured Product
  • KD/KO Validated

eRF3a/GSPT1 Polyclonal antibody

eRF3a/GSPT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28130-1-AP

Synonyms

GSPT1, eRF3a, EC:3.6.5.-, G1 to S phase transition 1, Eukaryotic peptide chain release factor GTP-binding subunit ERF3A

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using eRF3a/GSPT1 Polyclonal antibody (28130-1-AP)

    WB analysis of A549 using 28130-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 28130-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using eRF3a/GSPT1 Polyclonal antibody (28130-1-AP)

    WB analysis of HepG2 using 28130-1-AP

    WB result of eRF3a/GSPT1 antibody (28130-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-eRF3a/GSPT1 transfected HepG2 cells.

  • Western Blot (WB) analysis of PC-3 cells using eRF3a/GSPT1 Polyclonal antibody (28130-1-AP)

    WB analysis of PC-3 using 28130-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 28130-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using eRF3a/GSPT1 Polyclonal antibody (28130-1-AP)

    WB analysis of SKOV-3 using 28130-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 28130-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using eRF3a/GSPT1 Polyclonal antibody (28130-1-AP)

    IHC staining of human ovary cancer using 28130-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28130-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using eRF3a/GSPT1 Polyclonal antibody (28130-1-AP)

    IHC staining of human stomach using 28130-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28130-1-AP (eRF3a/GSPT1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using eRF3a/GSPT1 Polyclonal antibody (28130-1-AP)

    IF Staining of HeLa using 28130-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using eRF3a/GSPT1 antibody (28130-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, HepG2 cells, PC-3 cells, SKOV-3 cells, Hela cells
Positive IHC detected inhuman ovary cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28130-1-AP targets eRF3a/GSPT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen eRF3a/GSPT1 fusion protein Ag27963 相同性解析による交差性が予測される生物種
Full Name G1 to S phase transition 1
Calculated molecular weight 68 aa, 4 kDa
Observed molecular weight80 kDa
GenBank accession numberBC009503
Gene symbol GSPT1
Gene ID (NCBI) 2935
RRIDAB_2918149
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The eukaryotic Release Factor 3 (eRF3) is a GTPase that associates with eRF1 in a complex that mediates translation termination. Eukaryotic release factor 3 (eRF3) has many functions in eukaryotic cells, such as controlling the regulation of the cell cycle at the G1 to S phase transition, and regulating protein synthesis as a GTP dependent stimulator of eRF1 in translation termination. It was also reported to play a key role as an initiator of the mRNA degradation machinery in the recycling of ribosomes in successive cycles of translation,and probably also in transcription regulation. eRF3a, also known as GSPT1, is one subunit of eRF3(PMID:15917414,12923185). It involves in translation termination in response to the termination codons UAA, UAG and UGA and stimulates the activity of ERF1. eRF3a/GSPT1 exists some isoforms with MV 69 kDa and 56 kDa. Identification of a processed form of eRF3a/GSPT1 as a BIR3-binding protein-Using a GST-BIR3 fusion protein as an affinity reagent to purify new IAP binding proteins from extracts of human cells and mouse tissues, we previously isolated 3 proteins of molecular weights 23, 38 and 80 kDa. 80 kDa band confirmed that it is a processed form of the human GSPT1/eRF3a protein, lacking the first 69 residues(PMID: 12865429).

Protocols

Product Specific Protocols
WB protocol for eRF3a/GSPT1 antibody 28130-1-APDownload protocol
IHC protocol for eRF3a/GSPT1 antibody 28130-1-APDownload protocol
IF protocol for eRF3a/GSPT1 antibody 28130-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

bioRxiv

p300/CBP degradation is required to disable the active AR enhanceosome in prostate cancer

Authors - Jie Luo
View Article