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GSR Polyclonal antibody

Name: GSR Polyclonal antibody
Brand: Proteintech
SKU: 18257-1-AP
Rating: 5.00 (1 reviews)

GSR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 18257-1-AP

Synonyms

GRD1, GRase, glutathione reductase, GLUR, EC:1.8.1.7

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HeLa using 18257-1-AP
WB result of GSR antibody (18257-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSR transfected HeLa cells.
WB analysis using 18257-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18257-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 18257-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 18257-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 18257-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 18257-1-AP (GSR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 18257-1-AP
IP result of anti-GSR (IP:18257-1-AP, 4ug; Detection:18257-1-AP 1:500) with HeLa cells lysate 2600ug.
IHC staining of human breast cancer using 18257-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18257-1-AP (GSR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 18257-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18257-1-AP (GSR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 18257-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSR antibody (18257-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 18257-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells, mouse lung tissue, human placenta tissue, mouse testis tissue, rat testis tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 21 publications below
IF	See 1 publications below

Product Information

18257-1-AP targets GSR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GSR fusion protein Ag13080 相同性解析による交差性が予測される生物種
Full Name	glutathione reductase
Calculated molecular weight	522 aa, 56 kDa
Observed molecular weight	52-55 kDa
GenBank accession number	BC069244
Gene symbol	GSR
Gene ID (NCBI)	2936
RRID	AB_10598162
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GSR(Glutathione reductase) is also named as GLUR, GRD1 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.It was first found in a black American a variant red cell GSR characterized by greater electrophoretic mobility and enzyme activity per unit of hemoglobin than the normal.This protein maintains high levels of reduced glutathione in the cytosol.It has 5 isoforms produced by alternative splicing and alternative initiation.It can also exsit as a homodimer with the molecular weight of 110KDa(PMID:8631352).This antibody is specific to GSR.

Protocols

Product Specific Protocols
WB protocol for GSR antibody 18257-1-AP	Download protocol
IHC protocol for GSR antibody 18257-1-AP	Download protocol
IF protocol for GSR antibody 18257-1-AP	Download protocol
IP protocol for GSR antibody 18257-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Redox Biol Fast regulation of the NF-κB signalling pathway in human skeletal muscle revealed by high-intensity exercise and ischaemia at exhaustion: Role of oxygenation and metabolite accumulation. Authors - Angel Gallego-Selles View Article
mouse	WB	Cell Rep The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense. Authors - Wu Hongtan H View Article
human	WB	Nutrients A Mango Leaf Extract (Zynamite®) Combined with Quercetin Has Exercise-Mimetic Properties in Human Skeletal Muscle Authors - Miriam Martinez-Canton View Article
human	WB	Pharmacol Res Phyto-sesquiterpene lactones DET and DETD-35 induce ferroptosis in vemurafenib sensitive and resistant melanoma via GPX4 inhibition and metabolic reprogramming. Authors - Meng-Ting Chang View Article
human	WB	Free Radic Biol Med Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes. Authors - Kelei Dong View Article
mouse	WB	Free Radic Biol Med Antioxidant defense in quiescent cells determines selectivity of electron transport chain inhibition-induced cell death. Authors - Jan Blecha View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Hua (Verified Customer) (08-15-2024)

Good antibody. Very specific to GSR. Very clean background.

Applications: Western Blot
Primary Antibody Dilution: 1:2000
Cell Tissue Type: Mouse liver

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSR Polyclonal antibody

GSR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 18257-1-AP

WB analysis using 18257-1-AP

WB analysis of HeLa using 18257-1-AP

WB analysis of mouse lung using 18257-1-AP

WB analysis of human placenta using 18257-1-AP

WB analysis of Jurkat using 18257-1-AP

IP experiment of HeLa using 18257-1-AP

IHC staining of human breast cancer using 18257-1-AP

IHC staining of human breast cancer using 18257-1-AP

IF Staining of HeLa using 18257-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Fast regulation of the NF-κB signalling pathway in human skeletal muscle revealed by high-intensity exercise and ischaemia at exhaustion: Role of oxygenation and metabolite accumulation.

The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.

A Mango Leaf Extract (Zynamite®) Combined with Quercetin Has Exercise-Mimetic Properties in Human Skeletal Muscle

Phyto-sesquiterpene lactones DET and DETD-35 induce ferroptosis in vemurafenib sensitive and resistant melanoma via GPX4 inhibition and metabolic reprogramming.

Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.

Antioxidant defense in quiescent cells determines selectivity of electron transport chain inhibition-induced cell death.

Reviews