CoraLite® Plus 488-conjugated GST tag Monoclonal antibody

GST tag Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein, schistosoma japonicum

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B5F8

Cat no : CL488-66001

Synonyms

GST Tag



Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

CL488-66001 targets GST tag in WB applications and shows reactivity with recombinant protein, schistosoma japonicum samples.

Tested Reactivity recombinant protein, schistosoma japonicum
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Recombinant Protein 相同性解析による交差性が予測される生物種
Full Name GST tag
Calculated molecular weight 26 kDa
GenBank accession numberFN315687
Gene symbol
Gene ID (NCBI)
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 GST tag antibody CL488-66001Download protocol
Standard Protocols
Click here to view our Standard Protocols