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Validation Data Gallery

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  • Western Blot (WB) analysis of pig liver tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    WB analysis of pig liver using 66624-1-Ig (same clone as 66624-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    WB analysis of mouse liver using 66624-1-Ig (same clone as 66624-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    WB analysis of rat liver using 66624-1-Ig (same clone as 66624-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    WB analysis of human testis using 66624-1-Ig (same clone as 66624-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    WB analysis of human liver using 66624-1-Ig (same clone as 66624-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat kidney tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    WB analysis of rat kidney using 66624-1-Ig (same clone as 66624-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse hepatocytes using GSTA1 Monoclonal antibody (66624-1-Ig)

    WB analysis of mouse hepatocytes using 66624-1-Ig (same clone as 66624-1-PBS)

    mouse hepatocytes were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    IHC staining of human kidney using 66624-1-Ig (same clone as 66624-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    IHC staining of human kidney using 66624-1-Ig (same clone as 66624-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    IHC staining of human liver cancer using 66624-1-Ig (same clone as 66624-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    IHC staining of human liver cancer using 66624-1-Ig (same clone as 66624-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GSTA1 Monoclonal antibody (66624-1-Ig)

    IF Staining of HepG2 using 66624-1-Ig (same clone as 66624-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66624-1-Ig (GSTA1 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

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GSTA1 Monoclonal antibody, PBS Only

GSTA1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66624-1-PBS
Clone No.1E1F4

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Glutathione S transferase A1, GST class alpha member 1, GST epsilon, GST HA subunit 1, GST2, GSTA1, GSTA1 1, GTH1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66624-1-PBS targets GSTA1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GSTA1 fusion protein Ag6084 相同性解析による交差性が予測される生物種
Full Name glutathione S-transferase alpha 1
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC053578
Gene Symbol GSTA1
Gene ID (NCBI) 2938
RRIDAB_2881984
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08263
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.