Validation Data Gallery
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Tested Applications
| Positive WB detected in | human brain tissue |
| Positive IP detected in | SH-SY5Y cells |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IHC | See 1 publications below |
Product Information
17271-1-AP targets GSTA4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GSTA4 fusion protein Ag11028 相同性解析による交差性が予測される生物種 |
| Full Name | glutathione S-transferase alpha 4 |
| Calculated molecular weight | 222 aa, 27 kDa |
| Observed molecular weight | 27 kDa |
| GenBank accession number | BC015523 |
| Gene Symbol | GSTA4 |
| Gene ID (NCBI) | 2941 |
| RRID | AB_2295133 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15217 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GSTA4 antibody 17271-1-AP | Download protocol |
| IF protocol for GSTA4 antibody 17271-1-AP | Download protocol |
| IP protocol for GSTA4 antibody 17271-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. | ||
Free Radic Biol Med Increased hepatocellular protein carbonylation in human end-stage alcoholic cirrhosis. | ||
Mech Ageing Dev WDR23 mediates NRF2 proteostasis and cytoprotective capacity in the hippocampus | ||
J Lipid Res Susceptibility of L-FABP-/- mice to oxidative stress in early-stage alcoholic liver. | ||
PLoS One Short term feeding of a high fat diet exerts an additive effect on hepatocellular damage and steatosis in liver-specific PTEN knockout mice. | ||
Exp Mol Pathol Dysregulation of antioxidant responses in patients diagnosed with concomitant Primary Sclerosing Cholangitis/Inflammatory Bowel Disease. |