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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GSTM1 Polyclonal antibody (12412-1-AP)

    WB analysis using 12412-1-AP (same clone as 12412-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using GSTM1 Polyclonal antibody (12412-1-AP)

    WB analysis of mouse kidney using 12412-1-AP (same clone as 12412-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse ovary tissue using GSTM1 Polyclonal antibody (12412-1-AP)

    WB analysis of mouse ovary using 12412-1-AP (same clone as 12412-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GSTM1 Polyclonal antibody (12412-1-AP)

    WB analysis of HeLa using 12412-1-AP (same clone as 12412-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GSTM1 Polyclonal antibody (12412-1-AP)

    IHC staining of human gliomas using 12412-1-AP (same clone as 12412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GSTM1 Polyclonal antibody (12412-1-AP)

    IHC staining of human gliomas using 12412-1-AP (same clone as 12412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GSTM1 Polyclonal antibody (12412-1-AP)

    IHC staining of mouse liver using 12412-1-AP (same clone as 12412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GSTM1 Polyclonal antibody (12412-1-AP)

    IHC staining of mouse liver using 12412-1-AP (same clone as 12412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GSTM1 Polyclonal antibody (12412-1-AP)

    IF Staining of HeLa using 12412-1-AP (same clone as 12412-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GSTM1 antibody (12412-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

GSTM1 Polyclonal antibody, PBS Only

GSTM1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12412-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12412-1-PBS targets GSTM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSTM1 fusion protein Ag3113 相同性解析による交差性が予測される生物種
Full Name glutathione S-transferase mu 1
Calculated molecular weight 181 aa, 21 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC024005
Gene Symbol GSTM1
Gene ID (NCBI) 2944
RRIDAB_2115925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09488
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.