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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSTM1 Monoclonal antibody

GSTM1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, chicken

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3C5C2

Cat no : 67862-1-Ig

Synonyms

GST class-mu 1, GST class mu 1, Glutathione S-transferase Mu 1, glutathione S transferase mu 1, EC:2.5.1.18

Validation Data Gallery

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  • Western Blot (WB) analysis of rat liver tissue using GSTM1 Monoclonal antibody (67862-1-Ig)

    WB analysis of rat liver using 67862-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GSTM1 Monoclonal antibody (67862-1-Ig)

    WB analysis of HeLa using 67862-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using GSTM1 Monoclonal antibody (67862-1-Ig)

    WB analysis of Jurkat using 67862-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using GSTM1 Monoclonal antibody (67862-1-Ig)

    WB analysis of pig liver using 67862-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using GSTM1 Monoclonal antibody (67862-1-Ig)

    WB analysis of mouse liver using 67862-1-Ig

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of chicken liver tissue using GSTM1 Monoclonal antibody (67862-1-Ig)

    WB analysis of chicken liver using 67862-1-Ig

    chicken liver tissue were subjected to SDS PAGE followed by western blot with 67862-1-Ig (GSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GSTM1 Monoclonal antibody (67862-1-Ig)

    IF Staining of HeLa using 67862-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSTM1 antibody (67862-1-Ig, Clone: 3C5C2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using GSTM1 Monoclonal antibody (67862-1-Ig)

    FC experiment of HeLa using 67862-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GSTM1 (67862-1-Ig, Clone:3C5C2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inrat liver tissue, HeLa cells, Jurkat cells, pig liver tissue, mouse liver tissue, chicken liver tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67862-1-Ig targets GSTM1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, chicken samples.

Tested Reactivity human, mouse, rat, pig, chicken
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GSTM1 fusion protein Ag3113 相同性解析による交差性が予測される生物種
Full Name glutathione S-transferase mu 1
Calculated molecular weight 181 aa, 21 kDa
Observed molecular weight26-28 kDa
GenBank accession numberBC024005
Gene symbol GSTM1
Gene ID (NCBI) 2944
RRIDAB_2918620
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GSTM1 antibody 67862-1-IgDownload protocol
IF protocol for GSTM1 antibody 67862-1-IgDownload protocol
FC protocol for GSTM1 antibody 67862-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols