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KD/KO Validated

GSTM3 Polyclonal antibody

Name: GSTM3 Polyclonal antibody
Brand: Proteintech
SKU: 15214-1-AP

GSTM3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15214-1-AP

Synonyms

GST5, GST class-mu 3, GST class mu 3, Glutathione S-transferase Mu 3, Glutathione S transferase Mu 3

Validation Data Gallery

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AllWBIPIHC

WB analysis using 15214-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15214-1-AP (GSTM3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 15214-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 15214-1-AP (GSTM3 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 15214-1-AP
IP result of anti-GSTM3 (IP:15214-1-AP, 4ug; Detection:15214-1-AP 1:300) with mouse testis tissue lysate 4000ug.
IHC staining of mouse testis using 15214-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15214-1-AP (GSTM3 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 15214-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15214-1-AP (GSTM3 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 15214-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15214-1-AP (GSTM3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 15214-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15214-1-AP (GSTM3 antibody) at dilution of 1:100 (under 40x lens).

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Cat No. 15214-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse testis tissue, human testis tissue, rat testis tissue
Positive IP detected in	mouse testis tissue
Positive IHC detected in	mouse testis tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 16 publications below
IHC	See 7 publications below

Product Information

15214-1-AP targets GSTM3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, bovine, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GSTM3 fusion protein Ag7375 相同性解析による交差性が予測される生物種
Full Name	glutathione S-transferase mu 3 (brain)
Calculated molecular weight	27 kDa
Observed molecular weight	27-29 kDa
GenBank accession number	BC000088
Gene symbol	GSTM3
Gene ID (NCBI)	2947
RRID	AB_2116070
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GSTM3 is a cytosolic enzyme involved in prostaglandin and leukotriene synthesis and in the metabolization of toxic compounds, such as chemotherapeutic drugs, insecticides, herbicides, carcinogens, and by-products of oxidative stress. GSTM3 is expressed in testis, brain, lung, and lymphocytes. GSTM3 have been reported to be dysregulated in cancers like prostate cancer and colon cancer.

Protocols

Product Specific Protocols
WB protocol for GSTM3 antibody 15214-1-AP	Download protocol
IHC protocol for GSTM3 antibody 15214-1-AP	Download protocol
IP protocol for GSTM3 antibody 15214-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. Authors - Dong Huang View Article
human	WB	Mol Cell SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation Authors - Wenxin Zhang View Article
human	WB	Nat Chem Biol Enhanced mapping of small-molecule binding sites in cells Authors - Jacob M Wozniak View Article
		Cell Syst Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees. Authors - Stephanie A Zlatic View Article
human	WB,IHC	Cancer Lett GSTM3 deficiency impedes DNA mismatch repair to promote gastric tumorigenesis via CAND1/NRF2-KEAP1 signaling. Authors - Tao Chen KD Validated View Article
mouse	WB	Part Fibre Toxicol Protein target identification and toxicological mechanism investigation of silver nanoparticles-induced hepatotoxicity by integrating proteomic and metallomic strategies. Authors - Ming Xu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSTM3 Polyclonal antibody

GSTM3 Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis using 15214-1-AP

WB analysis of human testis using 15214-1-AP

IP experiment of mouse testis using 15214-1-AP

IHC staining of mouse testis using 15214-1-AP

IHC staining of mouse testis using 15214-1-AP

IHC staining of human testis using 15214-1-AP

IHC staining of human testis using 15214-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation

Enhanced mapping of small-molecule binding sites in cells

Rare Disease Mechanisms Identified by Genealogical Proteomics of Copper Homeostasis Mutant Pedigrees.

GSTM3 deficiency impedes DNA mismatch repair to promote gastric tumorigenesis via CAND1/NRF2-KEAP1 signaling.

Protein target identification and toxicological mechanism investigation of silver nanoparticles-induced hepatotoxicity by integrating proteomic and metallomic strategies.