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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSTO1 Monoclonal antibody, PBS Only

GSTO1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5E1E10

Cat no : 60150-2-PBS

Synonyms

glutathione S transferase omega 1, GSTO 1, GSTO1, P28

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GSTO1 Monoclonal antibody (60150-2-Ig)

    WB analysis using 60150-2-Ig (same clone as 60150-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using GSTO1 Monoclonal antibody (60150-2-Ig)

    WB analysis of human heart using 60150-2-Ig (same clone as 60150-2-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using GSTO1 Monoclonal antibody (60150-2-Ig)

    WB analysis of U-937 using 60150-2-Ig (same clone as 60150-2-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using GSTO1 Monoclonal antibody (60150-2-Ig)

    WB analysis of Raji using 60150-2-Ig (same clone as 60150-2-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using GSTO1 Monoclonal antibody (60150-2-Ig)

    WB analysis of ROS1728 using 60150-2-Ig (same clone as 60150-2-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using GSTO1 Monoclonal antibody (60150-2-Ig)

    WB analysis of NIH/3T3 using 60150-2-Ig (same clone as 60150-2-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60150-2-Ig (GSTO1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using GSTO1 Monoclonal antibody (60150-2-Ig)

    IHC staining of human oesophagus cancer using 60150-2-Ig (same clone as 60150-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using GSTO1 Monoclonal antibody (60150-2-Ig)

    IHC staining of human oesophagus cancer using 60150-2-Ig (same clone as 60150-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GSTO1 Monoclonal antibody (60150-2-Ig)

    IHC staining of human liver using 60150-2-Ig (same clone as 60150-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GSTO1 Monoclonal antibody (60150-2-Ig)

    IHC staining of human liver using 60150-2-Ig (same clone as 60150-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GSTO1 Monoclonal antibody (60150-2-Ig)

    IHC staining of human liver cancer using 60150-2-Ig (same clone as 60150-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GSTO1 Monoclonal antibody (60150-2-Ig)

    IHC staining of human liver cancer using 60150-2-Ig (same clone as 60150-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60150-2-Ig (GSTO1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using GSTO1 Monoclonal antibody (60150-2-Ig)

    IF Staining of PC-3 using 60150-2-Ig (same clone as 60150-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using GSTO1 antibody (60150-2-Ig, Clone: 5E1E10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60150-2-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60150-2-PBS targets GSTO1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GSTO1 fusion protein Ag7199 相同性解析による交差性が予測される生物種
Full Name glutathione S-transferase omega 1
Calculated molecular weight 28 kDa
Observed molecular weight 27-30 kDa
GenBank accession numberBC000127
Gene symbol GSTO1
Gene ID (NCBI) 9446
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Glutathione-S-transferase omega 1 (GSTO1) belongs to a new subfamily of GSTs and is the rate limiting enzyme for biotransformation of inorganic arsenic, environmental carcinogen. Expression of GSTO1-1 was abundant in a wide range of normal tissues, particularly liver, macrophages, glial cells, and endocrine cells.