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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GTDC1 Monoclonal antibody

GTDC1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C4C10

Cat no : 66578-1-Ig

Synonyms

GTDC1, mat Xa

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GTDC1 Monoclonal antibody (66578-1-Ig)

    WB analysis using 66578-1-Ig

    Various tissue were subjected to SDS PAGE followed by western blot with 66578-1-Ig (GTDC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using GTDC1 Monoclonal antibody (66578-1-Ig)

    IHC staining of human testis using 66578-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66578-1-Ig (GTDC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using GTDC1 Monoclonal antibody (66578-1-Ig)

    IHC staining of human testis using 66578-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66578-1-Ig (GTDC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using GTDC1 Monoclonal antibody (66578-1-Ig)

    IF Staining of U2OS using 66578-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 66578-1-Ig (GTDC1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inhuman cerebellum tissue, pig brain tissue, pig cerebellum tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66578-1-Ig targets GTDC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GTDC1 fusion protein Ag9934 相同性解析による交差性が予測される生物種
Full Name glycosyltransferase-like domain containing 1
Calculated molecular weight 292 aa, 34 kDa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC061699
Gene symbol GTDC1
Gene ID (NCBI) 79712
RRIDAB_2881938
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GTDC1 antibody 66578-1-IgDownload protocol
IHC protocol for GTDC1 antibody 66578-1-IgDownload protocol
IF protocol for GTDC1 antibody 66578-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols