GTF2B Polyclonal antibody
GTF2B Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, ELISA
Conjugate
Unconjugated
Cat no : 16467-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, HepG2 cells, HeLa cells, MCF-7 cells |
Positive IP detected in | K-562 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
Product Information
16467-1-AP targets GTF2B in WB, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GTF2B fusion protein Ag9547 相同性解析による交差性が予測される生物種 |
Full Name | general transcription factor IIB |
Calculated molecular weight | 316 aa, 35 kDa |
Observed molecular weight | 30-35 kDa |
GenBank accession number | BC020597 |
Gene symbol | GTF2B |
Gene ID (NCBI) | 2959 |
RRID | AB_2878263 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for GTF2B antibody 16467-1-AP | Download protocol |
IP protocol for GTF2B antibody 16467-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
PLoS Biol A novel TOX3-WDR5-ABCG2 signaling axis regulates the progression of colorectal cancer by accelerating stem-like traits and chemoresistance | ||
J Exp Clin Cancer Res Melatonin synergizes BRAF-targeting agent vemurafenib in melanoma treatment by inhibiting iNOS/hTERT signaling and cancer-stem cell traits. |