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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GTF2E1 Polyclonal antibody

GTF2E1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24405-1-AP

Synonyms

FE, GTF2E1, TF2E1, TFIIE A, TFIIE alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using GTF2E1 Polyclonal antibody (24405-1-AP)

    WB analysis of HEK-293 using 24405-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24405-1-AP (GTF2E1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using GTF2E1 Polyclonal antibody (24405-1-AP)

    IHC staining of rat kidney using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using GTF2E1 Polyclonal antibody (24405-1-AP)

    IHC staining of rat kidney using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using GTF2E1 Polyclonal antibody (24405-1-AP)

    IHC staining of mouse kidney using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using GTF2E1 Polyclonal antibody (24405-1-AP)

    IHC staining of mouse kidney using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GTF2E1 Polyclonal antibody (24405-1-AP)

    IHC staining of human stomach cancer using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GTF2E1 Polyclonal antibody (24405-1-AP)

    IHC staining of human stomach cancer using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using GTF2E1 Polyclonal antibody (24405-1-AP)

    IHC staining of human urothelial carcinoma using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using GTF2E1 Polyclonal antibody (24405-1-AP)

    IHC staining of human urothelial carcinoma using 24405-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 24405-1-AP (GTF2E1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman urothelial carcinoma tissue, rat kidney tissue, mouse kidney tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24405-1-AP targets GTF2E1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GTF2E1 fusion protein Ag19794 相同性解析による交差性が予測される生物種
Full Name general transcription factor IIE, polypeptide 1, alpha 56kDa
Calculated molecular weight 439 aa, 49 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC156997
Gene symbol GTF2E1
Gene ID (NCBI) 2960
RRIDAB_2879526
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GTF2E1 antibody 24405-1-APDownload protocol
IHC protocol for GTF2E1 antibody 24405-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols