Validation Data Gallery
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Tested Applications
| Positive WB detected in | K-562 cells, mouse ovary tissue, mouse thymus tissue, rat thymus tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15431-1-AP targets GTF2H4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GTF2H4 fusion protein Ag7680 相同性解析による交差性が予測される生物種 |
| Full Name | general transcription factor IIH, polypeptide 4, 52kDa |
| Calculated molecular weight | 52 kDa |
| Observed molecular weight | 45-50 kDa |
| GenBank accession number | BC004935 |
| Gene Symbol | GTF2H4 |
| Gene ID (NCBI) | 2968 |
| RRID | AB_3669207 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92759 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GTF2H4 (General Transcription Factor IIH Subunit 4) ,also named as P52, TFB2, TFIIH, is a component of the TFIIH complex, which plays a crucial role in transcription initiation by RNA polymerase II and is also involved in nucleotide excision repair (NER). The role of GTF2H4 in DNA repair mechanisms makes it a potentially important factor in the development and progression of cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GTF2H4 antibody 15431-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |