Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 10499-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10499-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10499-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10499-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10499-1-AP (TFII I antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human thyroid cancer using 10499-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10499-1-AP (TFII I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 10499-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10499-1-AP (TFII I antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 10499-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10499-1-AP (TFII I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse small intestine using 10499-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 10499-1-AP (TFII I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 10499-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10499-1-AP (TFII I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat small intestine using 10499-1-AP
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 10499-1-AP (TFII I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10499-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10499-1-AP (TFII I antibody at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 10499-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10499-1-AP (TFII I antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 10499-1-AP
Immunofluorescent analysis of Hela cells, using GTF2I antibody 10499-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

TFII I Polyclonal antibody

TFII I Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10499-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

BTKAP1, BTK associated protein 135, Bruton tyrosine kinase-associated protein 135, BAP-135, BAP135

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, Jurkat cells
Positive IHC detected in	human thyroid cancer tissue, human colon cancer tissue, human urothelial carcinoma tissue, mouse small intestine tissue, rat kidney tissue, rat small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IF	See 1 publications below

Product Information

10499-1-AP targets TFII I in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TFII I fusion protein Ag0773 相同性解析による交差性が予測される生物種
Full Name	general transcription factor II, i
Calculated molecular weight	112 kDa
Observed molecular weight	135 kDa
GenBank accession number	BC004472
Gene Symbol	TFII I
Gene ID (NCBI)	2969
RRID	AB_2114813
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P78347
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The GTF2I gene encodes a transcription factor. It negatively regulates agonist-induced calcium entry into cells by interfering with expression of the cation channel TRPC3 at the plasma membrane [PMID: 17023658]. As a transcription factor and/or as a regulator of intracellular calcium levels, GTF2I may play a role in the molecular basis of anxiety[PMID: 22578324]. It interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation [PMID: 16738337].

Protocols

Product Specific Protocols
WB protocol for TFII I antibody 10499-1-AP	Download protocol
IHC protocol for TFII I antibody 10499-1-AP	Download protocol
IF protocol for TFII I antibody 10499-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol Increased Nuclear Transporter Importin 7 Contributes to the Tumor Growth and Correlates With CD8 T Cell Infiltration in Cervical Cancer Authors - Jing Chen View Article
mouse	WB	Nature Post-translational control of beige fat biogenesis by PRDM16 stabilization Authors - Qiang Wang View Article
mouse	IF	iScience Characterizing the differential distribution and targets of Sumo1 and Sumo2 in the mouse brain Authors - Terry R Suk View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10499-1-AP

WB analysis of HEK-293 using 10499-1-AP

WB analysis of HeLa using 10499-1-AP

WB analysis of Jurkat using 10499-1-AP

IHC staining of human thyroid cancer using 10499-1-AP

IHC staining of human thyroid cancer using 10499-1-AP

IHC staining of human urothelial carcinoma using 10499-1-AP

IHC staining of mouse small intestine using 10499-1-AP

IHC staining of rat kidney using 10499-1-AP

IHC staining of rat small intestine using 10499-1-AP

IHC staining of human colon cancer using 10499-1-AP

IF Staining of HeLa using 10499-1-AP