GTF2IRD1 Polyclonal antibody

GTF2IRD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17052-1-AP

Synonyms

BEN, CREAM1, GTF2IRD1, GTF3, hMusTRD1alpha1, MUSTRD1, MusTRD1/BEN, RBAP2, USE B1 binding protein, WBS, WBSCR11, WBSCR12


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Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman testis tissue, human spleen tissue, human kidney tissue, human heart tissue, human ovary tissue, human skin tissue, human brain tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17052-1-AP targets GTF2IRD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GTF2IRD1 fusion protein Ag10718 相同性解析による交差性が予測される生物種
Full Name GTF2I repeat domain containing 1
Calculated molecular weight 959 aa, 106 kDa
Observed molecular weight 145 kDa, 120 kDa
GenBank accession numberBC018136
Gene symbol GTF2IRD1
Gene ID (NCBI) 9569
RRIDAB_2114956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GTF2IRD1 antibody 17052-1-APDownload protocol
IHC protocol for GTF2IRD1 antibody 17052-1-APDownload protocol
IF protocol for GTF2IRD1 antibody 17052-1-APDownload protocol
IP protocol for GTF2IRD1 antibody 17052-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols