Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 17052-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 17052-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 17052-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 17052-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 17052-1-AP
IP result of anti-GTF2IRD1 (IP:17052-1-AP, 4ug; Detection:17052-1-AP 1:500) with mouse liver tissue lysate 4000ug.
IHC staining of human testis using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skin using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human brain using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human heart using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HeLa using 17052-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

GTF2IRD1 Polyclonal antibody

GTF2IRD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17052-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

BEN, CREAM1, GTF2IRD1, GTF3, hMusTRD1alpha1, MUSTRD1, MusTRD1/BEN, RBAP2, USE B1 binding protein, WBS, WBSCR11, WBSCR12

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human testis tissue, human spleen tissue, human kidney tissue, human heart tissue, human ovary tissue, human skin tissue, human brain tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHC	See 1 publications below

Product Information

17052-1-AP targets GTF2IRD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GTF2IRD1 fusion protein Ag10718 相同性解析による交差性が予測される生物種
Full Name	GTF2I repeat domain containing 1
Calculated molecular weight	959 aa, 106 kDa
Observed molecular weight	145 kDa, 120 kDa
GenBank accession number	BC018136
Gene Symbol	GTF2IRD1
Gene ID (NCBI)	9569
RRID	AB_2114956
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UHL9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for GTF2IRD1 antibody 17052-1-AP	Download protocol
IHC protocol for GTF2IRD1 antibody 17052-1-AP	Download protocol
IF protocol for GTF2IRD1 antibody 17052-1-AP	Download protocol
IP protocol for GTF2IRD1 antibody 17052-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Int J Surg Single-cell and spatial transcriptomic analyses reveal heterogeneity characteristics and specific cell subtype regulators in growth hormone-secreting pituitary adenomas Authors - Yu Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 17052-1-AP

WB analysis of mouse liver using 17052-1-AP

WB analysis of mouse brain using 17052-1-AP

WB analysis of mouse kidney using 17052-1-AP

IP experiment of mouse liver using 17052-1-AP

IHC staining of human testis using 17052-1-AP

IHC staining of human skin using 17052-1-AP

IHC staining of human skin using 17052-1-AP

IHC staining of human brain using 17052-1-AP

IHC staining of human brain using 17052-1-AP

IHC staining of human liver using 17052-1-AP