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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GTF3C4 Polyclonal antibody (17653-1-AP)

    WB analysis of HeLa using 17653-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17653-1-AP (GTF3C4 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GTF3C4 Polyclonal antibody (17653-1-AP)

    WB analysis of HeLa using 17653-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17653-1-AP (GTF3C4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using GTF3C4 Polyclonal antibody (17653-1-AP)

    IP experiment of HeLa using 17653-1-AP

    IP result of anti-GTF3C4 (IP:17653-1-AP, 3ug; Detection:17653-1-AP 1:800) with HeLa cells lysate 875ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GTF3C4 Polyclonal antibody (17653-1-AP)

    IHC staining of human breast cancer using 17653-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17653-1-AP (GTF3C4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GTF3C4 Polyclonal antibody (17653-1-AP)

    IHC staining of human breast cancer using 17653-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17653-1-AP (GTF3C4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GTF3C4 Polyclonal antibody (17653-1-AP)

    IF Staining of HeLa using 17653-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17653-1-AP (GTF3C4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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GTF3C4 Polyclonal antibody

GTF3C4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 17653-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

GTF3C4, KAT12, TF3C delta, TFIII90, TFIIIC 90 kDa subunit, TFiiiC2 90, TFIIIC90, TFIIICdelta

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17653-1-AP targets GTF3C4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GTF3C4 fusion protein Ag11898 相同性解析による交差性が予測される生物種
Full Name general transcription factor IIIC, polypeptide 4, 90kDa
Calculated molecular weight 822 aa, 92 kDa
Observed molecular weight 92 kDa
GenBank accession numberBC094774
Gene Symbol GTF3C4
Gene ID (NCBI) 9329
RRIDAB_2115253
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKN8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GTF3C4, also named as TFIIIC90, is essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. It has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3.

Protocols

Product Specific Protocols
WB protocol for GTF3C4 antibody 17653-1-APDownload protocol
IHC protocol for GTF3C4 antibody 17653-1-APDownload protocol
IF protocol for GTF3C4 antibody 17653-1-APDownload protocol
IP protocol for GTF3C4 antibody 17653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Regulation of acetylation restores proteolytic function of diseased myocardium in mouse and human.

Authors - Ding Wang
View Article