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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using GTPBP1 Polyclonal antibody (16374-1-AP)

    WB analysis of mouse skeletal muscle using 16374-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16374-1-AP (GTPBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using GTPBP1 Polyclonal antibody (16374-1-AP)

    WB analysis of Jurkat using 16374-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16374-1-AP (GTPBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using GTPBP1 Polyclonal antibody (16374-1-AP)

    IP experiment of mouse skeletal muscle using 16374-1-AP

    IP result of anti-GTPBP1 (IP:16374-1-AP, 4ug; Detection:16374-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

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GTPBP1 Polyclonal antibody

GTPBP1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 16374-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

G protein 1, GP 1, GP1, GTP binding protein 1, GTPBP1, HSPC018

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, Jurkat cells
Positive IP detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16374-1-AP targets GTPBP1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GTPBP1 fusion protein Ag9332 相同性解析による交差性が予測される生物種
Full Name GTP binding protein 1
Calculated molecular weight 660 aa, 71 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC014075
Gene Symbol GTPBP1
Gene ID (NCBI) 9567
RRIDAB_2115267
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00178
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GTPBP1 antibody 16374-1-APDownload protocol
IP protocol for GTPBP1 antibody 16374-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols