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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GTPBP4 Polyclonal antibody (13897-1-AP)

    WB analysis using 13897-1-AP (same clone as 13897-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13897-1-AP (GTPBP4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using GTPBP4 Polyclonal antibody (13897-1-AP)

    WB analysis of mouse testis using 13897-1-AP (same clone as 13897-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13897-1-AP (GTPBP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using GTPBP4 Polyclonal antibody (13897-1-AP)

    WB analysis of human kidney using 13897-1-AP (same clone as 13897-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 13897-1-AP (GTPBP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using GTPBP4 Polyclonal antibody (13897-1-AP)

    IP experiment of HeLa using 13897-1-AP (same clone as 13897-1-PBS)

    IP result of anti-GTPBP4 (IP:13897-1-AP, 4ug; Detection:13897-1-AP 1:5000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using GTPBP4 Polyclonal antibody (13897-1-AP)

    IP experiment of mouse testis using 13897-1-AP (same clone as 13897-1-PBS)

    IP result of anti-GTPBP4 (IP:13897-1-AP, 4ug; Detection:13897-1-AP 1:800) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using GTPBP4 Polyclonal antibody (13897-1-AP)

    IF Staining of MDCK using 13897-1-AP (same clone as 13897-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using GTPBP4 antibody (13897-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

GTPBP4 Polyclonal antibody, PBS Only

GTPBP4 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 13897-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

NGB, GTP-binding protein NGB, GTP-binding protein 4, GTP binding protein NGB, GTP binding protein 4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13897-1-PBS targets GTPBP4 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GTPBP4 fusion protein Ag4859 相同性解析による交差性が予測される生物種
Full Name GTP binding protein 4
Calculated molecular weight 634 aa, 74 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC038975
Gene Symbol GTPBP4
Gene ID (NCBI) 23560
RRIDAB_2279486
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZE4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target.