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- KD/KO Validated
GTSE1 Polyclonal antibody
GTSE1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 21319-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, SH-SY5Y cells, MCF-7 cells, mouse liver tissue, K-562 cells, L02 cells, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 9 publications below |
| IHC | See 7 publications below |
| IF | See 2 publications below |
Product Information
21319-1-AP targets GTSE1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GTSE1 fusion protein Ag13497 相同性解析による交差性が予測される生物種 |
| Full Name | G-2 and S-phase expressed 1 |
| Calculated molecular weight | 77 kDa |
| Observed molecular weight | 66-77 kDa, 100 kDa |
| GenBank accession number | BC006325 |
| Gene symbol | GTSE1 |
| Gene ID (NCBI) | 51512 |
| RRID | AB_10733644 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GTSE1 is only expressed in the S and G2 phases of the cell cycle, where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage, the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53, shuttling it out of the nucleus and repressing its ability to induce apoptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GTSE1 antibody 21319-1-AP | Download protocol |
| IHC protocol for GTSE1 antibody 21319-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mod Pathol Expression profiling of small intestinal neuroendocrine tumors identifies subgroups with clinical relevance, prognostic markers and therapeutic targets. | ||
Mol Ther Nucleic Acids lncRNA LINC00665 Stabilized by TAF15 Impeded the Malignant Biological Behaviors of Glioma Cells via STAU1-Mediated mRNA Degradation. | ||
Cancer Sci High G2 and S-phase expressed 1 expression promotes acral melanoma progression and correlates with poor clinical prognosis. | ||
Lab Invest GTSE1 promotes tumor growth and metastasis by attenuating of KLF4 expression in clear cell renal cell carcinoma.
| ||
Lab Invest GTSE1 promotes prostate cancer cell proliferation via the SP1/FOXM1 signaling pathway.
| ||
Mol Carcinog G2 and S phase-expressed-1 induces chromosomal instability in esophageal squamous cell carcinoma cells and inhibits cell apoptosis through ROS/JNK signaling
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