Validation Data Gallery
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Tested Applications
| Positive WB detected in | U2OS cells, Saos-2 cells, HeLa cells, HEK-293 cells, MDA-MB-231 cells, Jurkat cells, K-562 cells, HSC-T6 cells, 4T1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68313-1-Ig targets GTSE1 in WB, ELISA applications and shows reactivity with Human, Rat, mouse samples.
| Tested Reactivity | Human, Rat, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GTSE1 fusion protein Ag32817 相同性解析による交差性が予測される生物種 |
| Full Name | G-2 and S-phase expressed 1 |
| Calculated molecular weight | 77 kDa |
| Observed molecular weight | 67 kDa |
| GenBank accession number | BC006325 |
| Gene Symbol | GTSE1 |
| Gene ID (NCBI) | 51512 |
| RRID | AB_2935391 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NYZ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GTSE1 is only expressed in the S and G2 phases of the cell cycle, where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage, the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53, shutting it out of the nucleus and repressing its ability to induce apoptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GTSE1 antibody 68313-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |