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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using Uroguanylin Polyclonal antibody (18113-1-AP)

    WB analysis of mouse pancreas using 18113-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat pancreas tissue using Uroguanylin Polyclonal antibody (18113-1-AP)

    WB analysis of rat pancreas using 18113-1-AP

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using Uroguanylin Polyclonal antibody (18113-1-AP)

    WB analysis of mouse colon using 18113-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using Uroguanylin Polyclonal antibody (18113-1-AP)

    WB analysis of mouse small intestine using 18113-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using Uroguanylin Polyclonal antibody (18113-1-AP)

    WB analysis of mouse small intestine using 18113-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 18113-1-AP (Uroguanylin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Uroguanylin Polyclonal antibody (18113-1-AP)

    IHC staining of human pancreas cancer using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Uroguanylin Polyclonal antibody (18113-1-AP)

    IHC staining of human pancreas cancer using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Uroguanylin Polyclonal antibody (18113-1-AP)

    IHC staining of human kidney using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Uroguanylin Polyclonal antibody (18113-1-AP)

    IHC staining of human kidney using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18113-1-AP (Uroguanylin antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Uroguanylin Polyclonal antibody (18113-1-AP)

    IHC staining of human kidney using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using Uroguanylin Polyclonal antibody (18113-1-AP)

    IHC staining of mouse small intestine using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Uroguanylin Polyclonal antibody (18113-1-AP)

    IHC staining of mouse kidney using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using Uroguanylin Polyclonal antibody (18113-1-AP)

    IHC staining of mouse small intestine using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Uroguanylin Polyclonal antibody (18113-1-AP)

    IHC staining of human colon cancer using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using Uroguanylin Polyclonal antibody (18113-1-AP)

    IHC staining of human stomach using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using Uroguanylin Polyclonal antibody (18113-1-AP)

    IHC staining of human stomach using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Uroguanylin Polyclonal antibody (18113-1-AP)

    IHC staining of human colon cancer using 18113-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18113-1-AP (Uroguanylin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Uroguanylin Polyclonal antibody (18113-1-AP)

    IF Staining of HepG2 using 18113-1-AP

    Immunofluorescent analysis of HepG2 cells using 18113-1-AP (Uroguanylin antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-rabbit IgG (red).

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Uroguanylin Polyclonal antibody

Uroguanylin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18113-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GUCA2B, UGN, Guanylate cyclase C-activating peptide 2, Guanylate cyclase activator 2B, GCAP II Uroguanylin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse pancreas tissue, mouse colon tissue, mouse small intestine tissue, rat pancreas tissue
Positive IHC detected inhuman pancreas cancer tissue, human kidney tissue, human stomach tissue, human colon cancer tissue, mouse small intestine tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18113-1-AP targets Uroguanylin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Uroguanylin fusion protein Ag12589 相同性解析による交差性が予測される生物種
Full Name guanylate cyclase activator 2B (uroguanylin)
Calculated molecular weight 112 aa, 12 kDa
Observed molecular weight 12-13 kDa
GenBank accession numberBC093779
Gene Symbol Uroguanylin
Gene ID (NCBI) 2981
RRIDAB_10858615
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16661
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Uroguanylin, also named as GUCA2B, is a candidate intestinal natriuretic hormone that controls the sodium and water balance between the intestine and the kidneys. It has been reported that the uroguanylin knockout mouse exhibits elevated blood pressure. Therefore, the GUCA2B gene is thought to be a susceptibility gene for essential hypertension (EH).

Protocols

Product Specific Protocols
WB protocol for Uroguanylin antibody 18113-1-APDownload protocol
IHC protocol for Uroguanylin antibody 18113-1-APDownload protocol
IF protocol for Uroguanylin antibody 18113-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Front Physiol

Decreased Colonic Guanylin/Uroguanylin Expression and Dried Stool Property in Mice With Social Defeat Stress.

Authors - Nobuhiko Ebisutani
View Article
humanWB

Hum Gene Ther

Gene Co-Expression and miRNA Regulation: A Path to Early Intervention in Colorectal Cancer

Authors - Jason C Huang
View Article