Featured Product
KD/KO Validated

GUCY1B3 Polyclonal antibody

Name: GUCY1B3 Polyclonal antibody
Brand: Proteintech
SKU: 19011-1-AP

GUCY1B3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 19011-1-AP

Synonyms

GCS-beta-1, GCS beta 3, GCS beta 1, GC SB3, GC S beta 1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 19011-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19011-1-AP (GUCY1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 19011-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 19011-1-AP (GUCY1B3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 19011-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 19011-1-AP (GUCY1B3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of human placenta using 19011-1-AP
IP result of anti-GUCY1B3 (IP:19011-1-AP, 4ug; Detection:19011-1-AP 1:500) with human placenta tissue lysate 2600 ug.
IHC staining of mouse brain using 19011-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19011-1-AP (GUCY1B3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 19011-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19011-1-AP (GUCY1B3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 19011-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19011-1-AP (GUCY1B3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 19011-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19011-1-AP (GUCY1B3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 19011-1-AP
Immunofluorescent analysis of Hela cells, using GUCY1B3 antibody 19011-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of MCF-7 using 19011-1-AP
Immunofluorescent analysis of MCF-7 cells, using GUCY1B3 antibody 19011-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

-/ -

Cat No. 19011-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human placenta tissue, mouse lung tissue, rat lung tissue
Positive IP detected in	human placenta tissue
Positive IHC detected in	mouse brain tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells, MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 2 publications below

Product Information

19011-1-AP targets GUCY1B3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GUCY1B3 fusion protein Ag5196 相同性解析による交差性が予測される生物種
Full Name	guanylate cyclase 1, soluble, beta 3
Calculated molecular weight	71 kDa
Observed molecular weight	67-71 kDa
GenBank accession number	BC047620
Gene symbol	GUCY1B3
Gene ID (NCBI)	2983
RRID	AB_2115817
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for GUCY1B3 antibody 19011-1-AP	Download protocol
IHC protocol for GUCY1B3 antibody 19011-1-AP	Download protocol
IF protocol for GUCY1B3 antibody 19011-1-AP	Download protocol
IP protocol for GUCY1B3 antibody 19011-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Commun Aortic disease in Marfan syndrome is caused by overactivation of sGC-PRKG signaling by NO. Authors - Andrea de la Fuente-Alonso View Article
mouse	WB	J Ethnopharmacol Modified Zhenwu Decoction improved intestinal barrier function of experimental colitis through activation of sGC-mediated cGMP/PKG signaling Authors - Yiqi Xu View Article
mouse	WB,IHC	Clin Sci (Lond) Empagliflozin prevents cardiomyopathy via sGC-cGMP-PKG pathway in type 2 diabetes mice. Authors - Mei Xue KD Validated View Article
mouse	WB,IHC	Sci Rep Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway. Authors - Catarina Rippe View Article
mouse	WB	Cell Tissue Res Cavin-3 (PRKCDBP) deficiency reduces the density of caveolae in smooth muscle. Authors - Baoyi Zhu View Article
rat	WB	World Neurosurg Upregulation of Connexin 40 Mediated by Nitric Oxide Attenuates Cerebral Vasospasm After Subarachnoid Hemorrhage via the Nitric Oxide-Cyclic Guanosine Monophosphate-Protein Kinase G Pathway. Authors - Shi Hai Lan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GUCY1B3 Polyclonal antibody

GUCY1B3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 19011-1-AP

WB analysis of human placenta using 19011-1-AP

WB analysis of mouse lung using 19011-1-AP

IP experiment of human placenta using 19011-1-AP

IHC staining of mouse brain using 19011-1-AP

IHC staining of mouse brain using 19011-1-AP

IHC staining of human lung cancer using 19011-1-AP

IHC staining of human lung cancer using 19011-1-AP

IF Staining of HeLa using 19011-1-AP

IF Staining of MCF-7 using 19011-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Aortic disease in Marfan syndrome is caused by overactivation of sGC-PRKG signaling by NO.

Modified Zhenwu Decoction improved intestinal barrier function of experimental colitis through activation of sGC-mediated cGMP/PKG signaling

Empagliflozin prevents cardiomyopathy via sGC-cGMP-PKG pathway in type 2 diabetes mice.

Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway.

Cavin-3 (PRKCDBP) deficiency reduces the density of caveolae in smooth muscle.

Upregulation of Connexin 40 Mediated by Nitric Oxide Attenuates Cerebral Vasospasm After Subarachnoid Hemorrhage via the Nitric Oxide-Cyclic Guanosine Monophosphate-Protein Kinase G Pathway.