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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GUCY2C Monoclonal antibody

GUCY2C Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F5F6

Cat no : 67782-1-Ig

Synonyms

GC C, Guanylyl cyclase C, GUC2C, GUCY2C, hSTAR, Intestinal guanylate cyclase, STA receptor, STAR

Validation Data Gallery

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  • Western Blot (WB) analysis of Caco-2 cells using GUCY2C Monoclonal antibody (67782-1-Ig)

    WB analysis of Caco-2 using 67782-1-Ig

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 67782-1-Ig (GUCY2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HaCaT cells using GUCY2C Monoclonal antibody (67782-1-Ig)

    WB analysis of HaCaT using 67782-1-Ig

    HaCaT cells were subjected to SDS PAGE followed by western blot with 67782-1-Ig (GUCY2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GUCY2C Monoclonal antibody (67782-1-Ig)

    WB analysis of HeLa using 67782-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67782-1-Ig (GUCY2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inCaco-2 cells, HaCaT cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67782-1-Ig targets GUCY2C in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GUCY2C fusion protein Ag19256 相同性解析による交差性が予測される生物種
Full Name guanylate cyclase 2C (heat stable enterotoxin receptor)
Calculated molecular weight 1073 aa, 123 kDa
Observed molecular weight130 kDa
GenBank accession numberBC136544
Gene symbol GUCY2C
Gene ID (NCBI) 2984
RRIDAB_2918546
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

GUCY2C is also named as GUC2C, STAR(Heat-stable enterotoxin receptor), Guanylyl cyclase C and belongs to the adenylyl cyclase class-4/guanylyl cyclase family. It is a receptor for the E.coli heat-stable enterotoxin and considered to be the true apical membrane receptor for the endogenous ligands uroguanylin and guanylin. GUCY2C is not present in the endocrine pancreas, but is localized exclusively to the ductal system which fully corresponds to the GUCY2C activity detected in the exocrine ductal system, but particularly not in islets of Langerhans. GUCY2C with a molecular mass of 130 kDa is detected in pancreatic and intestinal extracts.(PMID:11431160). The full length protein is 123 kDa and has a signal peptide and nine glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for GUCY2C antibody 67782-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols