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  • Featured Product
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GUF1 Polyclonal antibody

GUF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17489-1-AP

Synonyms

Translation factor GUF1, mitochondrial, Ribosomal back-translocase, GTPase GUF1, Elongation factor 4 homolog, EF-4

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using GUF1 Polyclonal antibody (17489-1-AP)

    WB analysis of HepG2 using 17489-1-AP

    WB result of GUF1 antibody (17489-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUF1 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using GUF1 Polyclonal antibody (17489-1-AP)

    WB analysis using 17489-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using GUF1 Polyclonal antibody (17489-1-AP)

    WB analysis of mouse brain using 17489-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GUF1 Polyclonal antibody (17489-1-AP)

    WB analysis of HepG2 using 17489-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using GUF1 Polyclonal antibody (17489-1-AP)

    WB analysis of mouse pancreas using 17489-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GUF1 Polyclonal antibody (17489-1-AP)

    WB analysis of HeLa using 17489-1-AP

    Hela cells were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using GUF1 Polyclonal antibody (17489-1-AP)

    IP experiment of HeLa using 17489-1-AP

    IP result of anti-GUF1 (IP:17489-1-AP, 3ug; Detection:17489-1-AP 1:500) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using GUF1 Polyclonal antibody (17489-1-AP)

    IHC staining of human oesophagus using 17489-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using GUF1 Polyclonal antibody (17489-1-AP)

    IHC staining of human oesophagus using 17489-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GUF1 Polyclonal antibody (17489-1-AP)

    IHC staining of human lung cancer using 17489-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GUF1 Polyclonal antibody (17489-1-AP)

    IHC staining of human lung cancer using 17489-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GUF1 Polyclonal antibody (17489-1-AP)

    IHC staining of human lung cancer using 17489-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GUF1 Polyclonal antibody (17489-1-AP)

    IHC staining of human lung cancer using 17489-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using GUF1 Polyclonal antibody (17489-1-AP)

    IHC staining of human kidney using 17489-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using GUF1 Polyclonal antibody (17489-1-AP)

    IHC staining of human kidney using 17489-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using GUF1 Polyclonal antibody (17489-1-AP)

    IHC staining of human testis using 17489-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using GUF1 Polyclonal antibody (17489-1-AP)

    IHC staining of human testis using 17489-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using GUF1 Polyclonal antibody (17489-1-AP)

    IHC staining of human oesophagus using 17489-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using GUF1 Polyclonal antibody (17489-1-AP)

    IHC staining of human oesophagus using 17489-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GUF1 Polyclonal antibody (17489-1-AP)

    IF Staining of HepG2 using 17489-1-AP

    Immunofluorescent analysis of HepG2 cells, using GUF1 antibody 17489-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, HepG2 cells, mouse pancreas tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman oesophagus tissue, human lung cancer tissue, human kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17489-1-AP targets GUF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GUF1 fusion protein Ag11579 相同性解析による交差性が予測される生物種
Full Name GUF1 GTPase homolog (S. cerevisiae)
Calculated molecular weight 669 aa, 74 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC036768
Gene symbol GUF1
Gene ID (NCBI) 60558
RRIDAB_2115960
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. In western blotting, we got double bands 74 kDa and 70 kDa, due to the N-terminal 1-49 amino acid sequence that could be cut down.

Protocols

Product Specific Protocols
WB protocol for GUF1 antibody 17489-1-APDownload protocol
IHC protocol for GUF1 antibody 17489-1-APDownload protocol
IF protocol for GUF1 antibody 17489-1-APDownload protocol
IP protocol for GUF1 antibody 17489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols