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  • Featured Product
  • KD/KO Validated

GUK1 Polyclonal antibody

GUK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10755-1-AP

Synonyms

GMK, GMP kinase, Guanylate kinase, guanylate kinase 1, GUK1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using GUK1 Polyclonal antibody (10755-1-AP)

    WB analysis of HEK-293 using 10755-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10755-1-AP (GUK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GUK1 Polyclonal antibody (10755-1-AP)

    WB analysis of HeLa using 10755-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10755-1-AP (GUK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GUK1 Polyclonal antibody (10755-1-AP)

    WB analysis of HEK-293 using 10755-1-AP

    WB result of GUK1 antibody (10755-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUK1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HeLa cells using GUK1 Polyclonal antibody (10755-1-AP)

    WB analysis of HeLa using 10755-1-AP

    WB result of GUK1 antibody (10755-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUK1 transfected HeLa cells.

  • Western Blot (WB) analysis of HEK-293 cells using GUK1 Polyclonal antibody (10755-1-AP)

    WB analysis of HEK-293 using 10755-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10755-1-AP (GUK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GUK1 Polyclonal antibody (10755-1-AP)

    WB analysis of HeLa using 10755-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10755-1-AP (GUK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GUK1 Polyclonal antibody (10755-1-AP)

    IHC staining of human kidney using 10755-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10755-1-AP (GUK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using GUK1 Polyclonal antibody (10755-1-AP)

    IHC staining of human kidney using 10755-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10755-1-AP (GUK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GUK1 Polyclonal antibody (10755-1-AP)

    IHC staining of human liver cancer using 10755-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10755-1-AP (GUK1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GUK1 Polyclonal antibody (10755-1-AP)

    IHC staining of human liver cancer using 10755-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10755-1-AP (GUK1 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10755-1-AP targets GUK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GUK1 fusion protein Ag1205 相同性解析による交差性が予測される生物種
Full Name guanylate kinase 1
Calculated molecular weight 22 kDa
Observed molecular weight 22-27 kDa
GenBank accession numberBC009914
Gene symbol GUK1
Gene ID (NCBI) 2987
RRIDAB_2115968
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GUK1 antibody 10755-1-APDownload protocol
IHC protocol for GUK1 antibody 10755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols