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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GULP1 Polyclonal antibody

GULP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19902-1-AP

Synonyms

PTB domain adapter protein CED-6, Protein GULP, GULP, Cell death protein 6 homolog, CED6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GULP1 Polyclonal antibody (19902-1-AP)

    WB analysis using 19902-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19902-1-AP (GULP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GULP1 Polyclonal antibody (19902-1-AP)

    WB analysis using 19902-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19902-1-AP (GULP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GULP1 Polyclonal antibody (19902-1-AP)

    IHC staining of human stomach cancer using 19902-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 19902-1-AP (GULP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells, HepG2 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19902-1-AP targets GULP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GULP1 fusion protein Ag13829 相同性解析による交差性が予測される生物種
Full Name GULP, engulfment adaptor PTB domain containing 1
Calculated molecular weight 167 aa, 19 kDa
Observed molecular weight34 kDa
GenBank accession numberBC001103
Gene symbol GULP1
Gene ID (NCBI) 51454
RRIDAB_10916254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GULP1 antibody 19902-1-APDownload protocol
IHC protocol for GULP1 antibody 19902-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Death Dis

The APC/C E3 ligase subunit ANAPC11 mediates FOXO3 protein degradation to promote cell proliferation and lymph node metastasis in urothelial bladder cancer

Authors - Dong Yan
View Article