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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GYG1 Polyclonal antibody

GYG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12836-1-AP

Synonyms

glycogenin 1, GN 1, GN1, GYG, GYG1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GYG1 Polyclonal antibody (12836-1-AP)

    WB analysis using 12836-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12836-1-AP (GYG1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using GYG1 Polyclonal antibody (12836-1-AP)

    WB analysis of human testis using 12836-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 12836-1-AP (GYG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using GYG1 Polyclonal antibody (12836-1-AP)

    IHC staining of human thyroid cancer using 12836-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 12836-1-AP (GYG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using GYG1 Polyclonal antibody (12836-1-AP)

    IHC staining of human thyroid cancer using 12836-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 12836-1-AP (GYG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using GYG1 Polyclonal antibody (12836-1-AP)

    IHC staining of mouse skeletal muscle using 12836-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12836-1-AP (GYG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using GYG1 Polyclonal antibody (12836-1-AP)

    IHC staining of rat skeletal muscle using 12836-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 12836-1-AP (GYG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inMCF-7 cells, human testis tissue, U-251 cells
Positive IHC detected inhuman thyroid cancer tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12836-1-AP targets GYG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GYG1 fusion protein Ag3583 相同性解析による交差性が予測される生物種
Full Name glycogenin 1
Calculated molecular weight 350 aa, 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC031096
Gene symbol GYG1
Gene ID (NCBI) 2992
RRIDAB_2116101
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GYG1 antibody 12836-1-APDownload protocol
IHC protocol for GYG1 antibody 12836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Carbohydr Polym

Liver fibrosis alters the molecular structures of hepatic glycogen

Authors - Yujun Wan
View Article