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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated GYPC Polyclonal antibody

GYPC Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-18147

Synonyms

CD236, CD236R, GE, GLPC, Glycoconnectin, Glycophorin C, Glycophorin D, Glycoprotein beta, GPC, GPD, GYPC, GYPD, PAS 2, Sialoglycoprotein D

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using CoraLite® Plus 488-conjugated GYPC Polyclonal anti (CL488-18147)

    IF Staining of K-562 using CL488-18147

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CoraLite® Plus 488 GYPC antibody (CL488-18147) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inK-562 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-18147 targets GYPC in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GYPC fusion protein Ag12825 相同性解析による交差性が予測される生物種
Full Name glycophorin C (Gerbich blood group)
Calculated molecular weight 128 aa, 14 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC106051
Gene symbol GYPC
Gene ID (NCBI) 2995
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycophorin-C, encoded by the GYPC gene, is a minor sialoglycoprotein in human erythrocyte membranes. Glycophorin-C interacts with protein 4.1R (EPB41) and plays an important role in regulating the mechanical stability of red cells. Glycophorin C acts as the receptor for the Plasmodium falciparum erythrocyte binding ligand PfEBP-2 (baebl). Glycophorin-D, also encoded by the GYPC gene, is now known as a variant of Glycophorin-C. This antibody is raised against the full-length protein of human Glycophorin-C. The apparent molecular weight of Glycophorin-C is larger than the calculated molecular weight due to glycosylation.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 GYPC antibody CL488-18147Download protocol
Standard Protocols
Click here to view our Standard Protocols