Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 22371-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22371-1-AP (GYS2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. Mouse heart/ kidney/ skeletal muscle are negative control.
WB analysis using 22371-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22371-1-AP (GYS2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 22371-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22371-1-AP (GYS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of rat liver using 22371-1-AP
IP result of anti-GYS2 (IP:22371-1-AP, 4ug; Detection:22371-1-AP 1:1000) with rat liver tissue lysate 4000 ug.
IHC staining of human liver using 22371-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22371-1-AP (GYS2 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 22371-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22371-1-AP (GYS2 antibody) at dilution of 1:200 (under 40x lens).

GYS2 Polyclonal antibody

GYS2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 22371-1-AP

Publications(15)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

glycogen synthase 2 (liver), Glycogen synthase 2, EC:2.4.1.11

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (15)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse liver tissue, mouse kidney tissue, rat liver tissue
Positive IP detected in	rat liver tissue
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 15 publications below
IHC	See 1 publications below

Product Information

22371-1-AP targets GYS2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GYS2 fusion protein Ag18012 相同性解析による交差性が予測される生物種
Full Name	glycogen synthase 2 (liver)
Calculated molecular weight	703 aa, 81 kDa
Observed molecular weight	81 kDa
GenBank accession number	BC126310
Gene Symbol	GYS2
Gene ID (NCBI)	2998
RRID	AB_2879091
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P54840
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for GYS2 antibody 22371-1-AP	Download protocol
IHC protocol for GYS2 antibody 22371-1-AP	Download protocol
IP protocol for GYS2 antibody 22371-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nature Glycogen metabolism links glucose homeostasis to thermogenesis in adipocytes. Authors - Omer Keinan View Article
rat	WB	Carbohydr Polym Liver fibrosis alters the molecular structures of hepatic glycogen Authors - Yujun Wan View Article
mouse	WB	Mol Metab Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction. Authors - Ferrol I Rome View Article
rat	WB	Food Funct l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways Authors - Ling Lin View Article
rat	WB	Metabolism Recurrent hypoglycemia increases hepatic gluconeogenesis without affecting glycogen metabolism or systemic lipolysis in rat Authors - Zejian Liu View Article
rat	WB	Nutrients Role of Epigallocatechin Gallate in Glucose, Lipid, and Protein Metabolism and L-Theanine in the Metabolism-Regulatory Effects of Epigallocatechin Gallate. Authors - Ling Lin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 22371-1-AP

WB analysis using 22371-1-AP

WB analysis using 22371-1-AP

IP experiment of rat liver using 22371-1-AP

IHC staining of human liver using 22371-1-AP

IHC staining of human liver using 22371-1-AP

GYS2 Polyclonal antibody

GYS2 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Glycogen metabolism links glucose homeostasis to thermogenesis in adipocytes.

Liver fibrosis alters the molecular structures of hepatic glycogen

Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction.

l-Theanine regulates glucose, lipid, and protein metabolism via insulin and AMP-activated protein kinase signaling pathways

Recurrent hypoglycemia increases hepatic gluconeogenesis without affecting glycogen metabolism or systemic lipolysis in rat

Role of Epigallocatechin Gallate in Glucose, Lipid, and Protein Metabolism and L-Theanine in the Metabolism-Regulatory Effects of Epigallocatechin Gallate.