Granzyme B Polyclonal antibody
Granzyme B Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 31521-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | NK-92 cells, U-937 cells |
Positive IHC detected in | human lymphoma tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:16000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
31521-1-AP targets Granzyme B in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
Full Name | granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) |
Calculated molecular weight | 247 aa, 28 kDa |
Observed molecular weight | 33 kDa |
GenBank accession number | BC030195 |
Gene symbol | Granzyme B |
Gene ID (NCBI) | 3002 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity Purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.
Protocols
Product Specific Protocols | |
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WB protocol for Granzyme B antibody 31521-1-AP | Download protocol |
IHC protocol for Granzyme B antibody 31521-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |