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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Granzyme B Polyclonal antibody

Granzyme B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31521-1-AP

Synonyms

CCPI, CGL 1, CGL1, CSP B, CSPB, CTLA1, CTSGL1, Endogenous granzyme B, GZMB, HLP, Homo sapiens (Human), SECT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GZMB Polyclonal antibody (31521-1-AP)

    WB analysis using 31521-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31521-1-AP (GZMB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inNK-92 cells, U-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31521-1-AP targets Granzyme B in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Calculated molecular weight 247 aa, 28 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC030195
Gene symbol GZMB
Gene ID (NCBI) 3002
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.

Protocols

Product Specific Protocols
WB protocol for Granzyme B antibody 31521-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols